Belachew Tesfaye, Mulusew Eyuel, Tolosa Yonas, Asefa Zerihun, Negussie Haileleul, Sori Teshale
Epidemiology Unit, Asella Regional Veterinary Laboratory, Asella, Oromia State, Ethiopia.
Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Oromia State, Ethiopia.
Infect Drug Resist. 2021 Sep 29;14:4047-4055. doi: 10.2147/IDR.S331249. eCollection 2021.
Salmonellosis is a foodborne zoonoses found worldwide. The main purpose of this study was to isolate and identify and assess their antimicrobial susceptibility profiles from smallhold broilers supply chains and slaughterhouses in Bishoftu and Modjo, central Ethiopia.
Four smallhold broiler farms under the auspices of Chico Meat were selected randomly. Feed, water, and water- and feed-trough samples were collected from broiler farms, while cecal contents were collected from slaughtered chicken at Chico Meat slaughterhouse. Conventional bacteriological techniques were used to isolate and identify from the samples. Kirby-Bauer disk diffusion was employed to assess the antimicrobial susceptibility of the isolates.
was isolated from 131 (24.3%, 95% CI 20.74-28.15) of the 539 samples tested. was found in 43 of the 250 samples collected from Bishoftu (22%, 95% CI 17.02%-27.65%) and 76 of the 289 samples collected from Modjo (26.29%, 95% CI 21.32%-31.77%). was isolated from 26.46% of the cloacal samples, 21% of the cecal contents, 30.77% of the feed samples, 25% of the water samples, 22.22% of samples from feed troughs, and 20% of samples from water troughs. The highest level of resistance (80.81%) was observed against tetracycline, followed by kanamycin (71.72%), chloramphenicol and amoxicillin (67.68%), sulfamethazole-trimethoprim (61.62%), naldixic acid (63.64%), and streptomycin (59.60%), whereas most of the isolates were susceptible to gentamicin (69.70%). Resistance to more than two drugs was also observed.
was found in high prevalence in broilers, their feed, and their environment. Moreover, a majority of the isolates were resistant to most antimicrobials used in medical and poultry practices. This has significant implications for public health and antimicrobial resistance.
沙门氏菌病是一种在全球范围内发现的食源性人畜共患病。本研究的主要目的是从埃塞俄比亚中部比绍夫图和莫乔的小规模肉鸡供应链及屠宰场中分离、鉴定沙门氏菌，并评估其抗菌药敏谱。
随机选择了在奇科肉类公司支持下的四个小规模肉鸡养殖场。从肉鸡养殖场采集饲料、水以及水槽和料槽样本，同时在奇科肉类屠宰场从屠宰的鸡中采集盲肠内容物。采用常规细菌学技术从样本中分离和鉴定沙门氏菌。采用 Kirby-Bauer 纸片扩散法评估分离株的抗菌药敏情况。
在检测的 539 个样本中，有 131 个（24.3%，95%可信区间 20.74 - 28.15）分离出了沙门氏菌。在从比绍夫图采集的 250 个样本中有 43 个（22%，95%可信区间 17.02% - 27.65%）检测到沙门氏菌，在从莫乔采集的 289 个样本中有 76 个（26.29%，95%可信区间 21.32% - 31.77%）检测到沙门氏菌。从 26.46%的泄殖腔样本、21%的盲肠内容物样本、30.77%的饲料样本、25%的水样、22.22%的料槽样本和 20%的水槽样本中分离出了沙门氏菌。观察到对四环素的耐药水平最高（80.81%），其次是卡那霉素（71.72%）、氯霉素和阿莫西林（67.68%）、磺胺甲恶唑 - 甲氧苄啶（61.62%）、萘啶酸（63.64%）和链霉素（59.60%），而大多数分离株对庆大霉素敏感（69.70%）。还观察到对两种以上药物的耐药情况。
在肉鸡及其饲料和环境中沙门氏菌的检出率很高。此外，大多数分离株对医疗和家禽实践中使用的大多数抗菌药物耐药。这对公共卫生和抗菌药物耐药性具有重要影响。
