磷酸镁铵复合载细胞水凝胶促进成骨和血管生成
Magnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis .
作者信息
Liu Chang, Yang Guangzheng, Zhou Mingliang, Zhang Xiangkai, Wu Xiaolin, Wu Peishi, Gu Xiaoyu, Jiang Xinquan
机构信息
Department of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
出版信息
ACS Omega. 2021 Apr 2;6(14):9449-9459. doi: 10.1021/acsomega.0c06083. eCollection 2021 Apr 13.
Injectable hydrogels provide an effective strategy for minimally invasive treatment on irregular bony defects in the maxillofacial region. To improve the osteoinduction of gelatin methacrylate (GelMA), we fabricated a three-dimensional (3D) culture system based on the incorporation of magnesium ammonium phosphate hexahydrate (struvite) into GelMA. The optimal concentration of struvite was investigated using the struvite extracts, and 500 μg mL was found to be the most suitable concentration for the osteogenesis of dental pulp stem cells (DPSCs) and angiogenesis of human umbilical vein endothelial cells (HUVECs). We prepared the GelMA composite (MgP) with 500 μg mL struvite. Struvite did not affect the cross-linking of GelMA and released Mg during degradation. The cell delivery system using MgP improved the laden-cell viability, upregulated the expression of osteogenic and angiogenic-differentiation-related genes, and promoted cell migration. Overall, the modifications made to the GelMA in this study improved osteoinduction and demonstrated great potential for application in vascularized bone tissue regeneration.
可注射水凝胶为颌面区域不规则骨缺损的微创治疗提供了一种有效策略。为提高甲基丙烯酸明胶（GelMA）的骨诱导能力，我们通过将六水合磷酸镁铵（鸟粪石）掺入GelMA中构建了一种三维（3D）培养系统。使用鸟粪石提取物研究了鸟粪石的最佳浓度，发现500μg/mL是牙髓干细胞（DPSC）成骨和人脐静脉内皮细胞（HUVEC）血管生成的最合适浓度。我们制备了含500μg/mL鸟粪石的GelMA复合材料（MgP）。鸟粪石不影响GelMA的交联，并在降解过程中释放镁。使用MgP的细胞递送系统提高了负载细胞的活力，上调了成骨和血管生成分化相关基因的表达，并促进了细胞迁移。总体而言，本研究中对GelMA所做的修饰改善了骨诱导能力，并在血管化骨组织再生中显示出巨大的应用潜力。
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