Cui Jingjing, Fu Yuming, Yi Zhihao, Dong Chen, Liu Hong
Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing, 100191, China.
Appl Microbiol Biotechnol. 2021 May;105(9):3705-3715. doi: 10.1007/s00253-021-11282-2. Epub 2021 Apr 24.
The general public spends one-third of its time under artificial lighting, which lacks bands beneficial to human health, and long-term exposure will have a negative impact on bone health. Here, we report the effects of long-term, low-dose ultraviolet (UV) supplementation to white light-emitting diode (LED) light exposure on intestinal microorganisms and bone metabolism, as well as the correlations between the two. Normal and ovariectomized rats were irradiated with LED white light with or without supplementation with UV. The effects of UV supplementation on the intestinal flora and the relationship between the intestinal flora and bone were investigated by measuring the intestinal flora, bone metabolism markers, and bone histomorphology. UV supplementation affected the bone density and bone mass by changing the relative content of Firmicutes, Saccharibacteria, and Proteobacteria; however, the intestinal flora were not the only factors affecting bone. Ultraviolet supplementation changed the composition and function of the gut flora in the bone loss model. By increasing the synthesis of short-chain fatty acids and affecting immunomodulatory, intestinal flora directly or indirectly regulate the activity of osteoclasts and thus mediate UV-mediated improvements in bone metabolism. Our work shows that UV supplementation affects bone density by influencing the intestinal flora, introducing a novel strategy to develop healthier artificial light sources and prevent bone loss. KEY POINTS: • We measured the bone metabolism markers and bone histomorphometry of rats. • The diversity, composition, and function of intestinal flora were analyzed. • The relationship between gut microbiota and host bone physiology was analyzed.
普通大众有三分之一的时间处于人造光下，而人造光缺乏对人体健康有益的波段，长期暴露会对骨骼健康产生负面影响。在此，我们报告长期低剂量补充紫外线（UV）对白光发光二极管（LED）光照下肠道微生物群和骨代谢的影响，以及二者之间的相关性。对正常和去卵巢大鼠照射有无补充UV的LED白光。通过测量肠道菌群、骨代谢标志物和骨组织形态学，研究补充UV对肠道菌群的影响以及肠道菌群与骨骼之间的关系。补充UV通过改变厚壁菌门、糖菌门和变形菌门的相对含量影响骨密度和骨量；然而，肠道菌群并非影响骨骼的唯一因素。补充紫外线改变了骨质流失模型中肠道菌群的组成和功能。通过增加短链脂肪酸的合成并影响免疫调节，肠道菌群直接或间接调节破骨细胞的活性，从而介导UV对骨代谢的改善作用。我们的研究表明，补充UV通过影响肠道菌群来影响骨密度，为开发更健康的人造光源和预防骨质流失引入了一种新策略。要点：• 我们测量了大鼠的骨代谢标志物和骨组织形态计量学。• 分析了肠道菌群的多样性、组成和功能。• 分析了肠道微生物群与宿主骨骼生理学之间的关系。
