Park Min-Gyu, Kim In-Ryoung, Kim Hyung Joon, Kwak Sang Won, Kim Hyeon-Cheol
Department of Conservative Dentistry, Dental Research Institute, Pusan National University School of Dentistry, Yangsan, Korea.
Department of Oral Anatomy, Pusan National University School of Dentistry, Yangsan, Korea.
Aust Endod J. 2021 Dec;47(3):512-519. doi: 10.1111/aej.12515. Epub 2021 Apr 24.
The purpose of this study was to compare the physical properties and cytocompatibility of contemporary calcium silicate-based sealers. Four calcium silicate-based sealers (BrightEndo MTA sealer, CeraSeal, EndoSeal TCS and One-Fil) were compared to an epoxy resin-based sealer (AH Plus). Flow, setting time, radiopacity and dimensional change were evaluated according to ISO 6876 standards. Cytotoxicity on human periodontal ligament fibroblast (hPDLF) cells was compared for biological properties using MTT assay. The surface of the sealer was analysed using scanning electron microscopy to evaluate cell attachment. Flow and radiopacity of all sealers met ISO standards, while setting time and dimensional stability did not meet the ISO standards. Calcium silicate-based sealers showed favourable cytocompatibility, and hPDLF cells were well attached to the calcium silicate-based sealers. Calcium silicate-based sealers have clinically acceptable flow and radiopacity, and cytocompatibility. However, these sealers had longer setting time and higher dimensional change than those required by ISO 6876.
本研究的目的是比较当代硅酸钙基封闭剂的物理性能和细胞相容性。将四种硅酸钙基封闭剂（BrightEndo MTA封闭剂、CeraSeal、EndoSeal TCS和One-Fil）与一种环氧树脂基封闭剂（AH Plus）进行比较。根据ISO 6876标准评估流动性、凝固时间、射线不透性和尺寸变化。使用MTT法比较对人牙周膜成纤维细胞（hPDLF）的细胞毒性以评估生物学性能。使用扫描电子显微镜分析封闭剂表面以评估细胞附着情况。所有封闭剂的流动性和射线不透性均符合ISO标准，而凝固时间和尺寸稳定性不符合ISO标准。硅酸钙基封闭剂显示出良好的细胞相容性，并且hPDLF细胞能很好地附着在硅酸钙基封闭剂上。硅酸钙基封闭剂具有临床上可接受的流动性、射线不透性和细胞相容性。然而，这些封闭剂的凝固时间比ISO 6876要求的更长，尺寸变化更大。
