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藤茎内生真菌的分子系统发育及其对植物病原真菌的拮抗活性

Molecular Phylogeny of Endophytic Fungi from Rattan ( Griff.) Spines and Their Antagonistic Activities against Plant Pathogenic Fungi.

作者信息

Azuddin Nurul Farizah, Mohd Masratul Hawa, Rosely Nik Fadzly N, Mansor Asyraf, Zakaria Latiffah

机构信息

School of Biological Sciences, Universiti Sains Malaysia, Penang USM 11800, Malaysia.

出版信息

J Fungi (Basel). 2021 Apr 15;7(4):301. doi: 10.3390/jof7040301.

Abstract

is a common rattan palm species in the tropical forests of Peninsular Malaysia and is noticeable by the yellow-based spines that cover the stems. This study aimed to determine the prevalence of fungal endophytes within spines and whether they inhibit the growth of fungal pathogens. Twenty-one genera with 40 species of fungal endophytes were isolated and identified from rattan palm spines. Based on molecular identification, the most common isolates recovered from the spines were ( 19) and spp. ( 18), followed by spp., sp., spp., spp., spp., spp., spp., sp., sp., spp., sp., sp., and spp. Non-sporulating fungi were also identified, namely , , , , , and . The isolation of these endophytes showed that the spines harbor endophytic fungi. Most of the fungal endophytes inhibited the growth of several plant pathogenic fungi, with 68% of the interactions resulting in mutual inhibition, producing a clear inhibition zone of <2 mm. Our findings demonstrate the potential of the fungal endophytes from spines as biocontrol agents.

摘要

是马来西亚半岛热带森林中常见的藤本棕榈物种,其茎上覆盖着黄色基部的刺,十分引人注目。本研究旨在确定刺中真菌内生菌的患病率,以及它们是否抑制真菌病原体的生长。从藤本棕榈刺中分离并鉴定出了21个属40种真菌内生菌。基于分子鉴定,从刺中回收的最常见分离株是(19)和 spp.(18),其次是 spp.、 sp.、 spp.、 spp.、 spp.、 spp.、 spp.、 sp.、 sp.、 spp.、 sp.、 sp.和 spp.。还鉴定出了非产孢真菌,即 、 、 、 、 和 。这些内生菌的分离表明刺中含有内生真菌。大多数真菌内生菌抑制了几种植物病原真菌的生长,68% 的相互作用导致相互抑制,产生了 <2 mm 的明显抑制区。我们的研究结果证明了来自 刺的真菌内生菌作为生物防治剂的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74be/8071255/f3fca68a49c5/jof-07-00301-g001.jpg

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