Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Universitätsklinikum Erlangen, 91054 Erlangen, Germany.
Department of Internal Medicine 3-Rheumatology and Immunology, Universitätsklinikum Erlangen and Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany.
Int J Mol Sci. 2021 Apr 16;22(8):4143. doi: 10.3390/ijms22084143.
Hydroxyapatite- or calcium phosphate-coated iron oxide nanoparticles have a high potential for use in many biomedical applications. In this study, a co-precipitation method for the synthesis of hydroxyapatite-coated nanoparticles (SPION), was used. The produced nanoparticles have been characterized by dynamic light scattering, X-ray diffraction, vibrating sample magnetometry, Fourier transform infrared spectrometry, atomic emission spectroscopy, scanning electron microscopy, transmission electron microscopy, selected area diffraction, and energy-dispersive X-ray spectroscopy. The results showed a successful synthesis of 190 nm sized particles and their stable coating, resulting in SPION. Potential cytotoxic effects of SPION on EL4, THP-1, and Jurkat cells were tested, showing only a minor effect on cell viability at the highest tested concentration (400 µg Fe/mL). The results further showed that hydroxyapatite-coated SPIONs can induce minor TNF-α and IL-6 release by murine macrophages at a concentration of 100 µg Fe/mL. To investigate if and how such particles interact with other substances that modulate the immune response, SPION-treated macrophages were incubated with LPS (lipopolysaccharides) and dexamethasone. We found that cytokine release in response to these potent pro- and anti-inflammatory agents was modulated in the presence of SPION. Knowledge of this behavior is important for the management of inflammatory processes following in vivo applications of this type of SPIONs.
羟基磷灰石或磷酸钙涂层的氧化铁纳米粒子在许多生物医学应用中具有很高的应用潜力。在这项研究中，采用共沉淀法合成了羟基磷灰石涂层的纳米粒子（SPION）。通过动态光散射、X 射线衍射、振动样品磁强计、傅里叶变换红外光谱、原子发射光谱、扫描电子显微镜、透射电子显微镜、选区衍射和能谱对所制备的纳米粒子进行了表征。结果表明，成功合成了 190nm 大小的颗粒，并对其进行了稳定的涂层处理，得到了 SPION。测试了 SPION 对 EL4、THP-1 和 Jurkat 细胞的潜在细胞毒性作用，结果表明，在最高测试浓度（400μg Fe/mL）下，对细胞活力的影响很小。结果还表明，羟基磷灰石涂层的 SPION 在 100μg Fe/mL 的浓度下可以诱导小鼠巨噬细胞释放少量 TNF-α 和 IL-6。为了研究这些颗粒是否以及如何与其他调节免疫反应的物质相互作用，用 SPION 处理的巨噬细胞与 LPS（脂多糖）和地塞米松孵育。我们发现，在存在 SPION 的情况下，这些强效促炎和抗炎剂的细胞因子释放被调节。了解这种行为对于管理体内应用这种类型的 SPION 后炎症过程非常重要。
