State Key laboratory of Oncology in South China, Collaborative innovation Center for cancer Medicine, Guangzhou, P. R. China.
Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
BMC Cancer. 2021 Apr 30;21(1):479. doi: 10.1186/s12885-021-08234-4.
Ubiquitin-conjugating enzyme E2W (UBE2W) is a protein-coding gene that has an important role in ubiquitination and may be vital in the repair of DNA damage. However, studies on the prognostic value of UBE2W and its correlation with tumor-infiltrating immune cells in multiple cancers have not been addressed.
We investigated UBE2W expression in the Oncomine database, the Tumor Immune Estimation Resource (TIMER), TNMplot database. Then, the clinical prognostic value of UBE2W was analyzed via online public databases. Meanwhile, we explored the correlation between UBE2W and DNA repair associate genes expression and DNA methyltransferase expression by TIMER and Gene Expression Profiling Interactive Analysis (GEPIA). By using the same method, the correlation between UBE2W and tumor-infiltrating immune cells was explored. Genomic Profiles of UBE2W in breast cancer (BRCA) were accessed in cBioPortal (v3.5.0). Functional proteins associated network was analyzed by STRING database (v11.0).
UBE2W was abnormally expressed and significantly correlated with mismatch repair (MMR) gene mutation levels, DNA methyltransferase, and BRCA1/2 expression in breast cancer. High expression of UBE2W may promote the tumor immunosuppression and metastasis, induce endocrine therapy resistance and deteriorate outcomes of patients with breast cancer. These findings suggest that UBE2W could be a potential biomarker of prognosis and tumor-infiltrating immune cells. Besides, RBX1 may be a new E3 that was regulated by UBE2W.
Ubiquitin E2 UBE2W is a potential prognostic biomarker and is correlated with immune infiltration in BRCA.
泛素结合酶 E2W（UBE2W）是一种蛋白质编码基因，在泛素化过程中发挥重要作用，可能对 DNA 损伤的修复至关重要。然而，UBE2W 在多种癌症中的预后价值及其与肿瘤浸润免疫细胞的相关性研究尚未得到解决。
我们在 Oncomine 数据库、肿瘤免疫估计资源（TIMER）和 TNMplot 数据库中研究了 UBE2W 的表达。然后，通过在线公共数据库分析 UBE2W 的临床预后价值。同时，我们通过 TIMER 和基因表达谱交互分析（GEPIA）探讨了 UBE2W 与 DNA 修复相关基因表达和 DNA 甲基转移酶表达的相关性。同样的方法，我们探讨了 UBE2W 与肿瘤浸润免疫细胞的相关性。在 cBioPortal（v3.5.0）中访问了乳腺癌（BRCA）中 UBE2W 的基因组图谱。通过 STRING 数据库（v11.0）分析了功能蛋白相关网络。
UBE2W 异常表达，与乳腺癌中错配修复（MMR）基因突变水平、DNA 甲基转移酶和 BRCA1/2 表达显著相关。UBE2W 的高表达可能促进肿瘤免疫抑制和转移，诱导内分泌治疗耐药，并恶化乳腺癌患者的结局。这些发现表明 UBE2W 可能是一个潜在的预后和肿瘤浸润免疫细胞的生物标志物。此外，RBX1 可能是一个受 UBE2W 调控的新的 E3。
泛素 E2 UBE2W 是 BRCA 中一种潜在的预后生物标志物，与免疫浸润相关。
