Truong Quang Lam, Nguyen Lan Thi, Babikian Haig Yousef, Jha Rajeev Kumar, Nguyen Hoa Thi, To Thanh Long
Key Laboratory of Veterinary Biotechnology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
Department of Research and Development, PT. Rhea Natural Sciences, Jakarta, Indonesia.
Vet World. 2021 Mar;14(3):794-802. doi: 10.14202/vetworld.2021.794-802. Epub 2021 Mar 30.
African swine fever is one of the severe pathogens of swine. It has a significant impact on production and economics. So far, there are no known remedies, such as vaccines or drugs, reported working successfully. In the present study, the natural oil blend formulation's (NOBF) efficacy was evaluated against ASFV o using porcine alveolar macrophages (PAMs) cells of swine.
The capacity of NOBF against the ASFV was tested . The NOBF combines , , and . We used a 2-fold serial dilution to test the NOBF formulation dose, that is, 10 HAD50/mL, against purified lethal dose of African swine in primary PAMs cells of swine. The PAM cells survival, real-time polymerase chain reaction (PCR) test, and hemadsorption (HAD) observation were performed to check the NOBF efficacy against ASFV.
The trial results demonstrated that NOBF up to dilution 13 or 0.000625 mL deactivates the lethal dose 10 HAD50 of ASFV. There was no HAD (Rosetta formation) up to dilution 12 or 0.00125 mL of NOBF. The Ct value obtained by running real-time PCR of the NOBF group at 96 h post-infection was the same as the initial value or lower (25), whereas the Ct value of positive controls increased several folds (17.84).
The trial demonstrated that NOBF could deactivate the ASFV. The NOBF has the potential to act as anti-ASFV agent in the field. The next step is to conduct level trial to determine its efficacy.
非洲猪瘟是猪的严重病原体之一。它对生产和经济有重大影响。迄今为止，尚无已知的补救措施，如疫苗或药物被报道能成功发挥作用。在本研究中，使用猪的肺泡巨噬细胞（PAMs）对天然油混合制剂（NOBF）针对非洲猪瘟病毒（ASFV）的功效进行了评估。
测试了NOBF对ASFV的作用能力。NOBF由[具体成分1]、[具体成分2]和[具体成分3]混合而成。我们采用2倍系列稀释法，在猪的原代PAMs细胞中测试NOBF制剂剂量（即10 HAD50/mL）对纯化的非洲猪瘟致死剂量的作用。进行PAM细胞存活、实时聚合酶链反应（PCR）测试和血细胞吸附（HAD）观察，以检查NOBF对ASFV的功效。
试验结果表明，稀释至13倍或0.000625 mL的NOBF可使10 HAD50的ASFV致死剂量失活。稀释至12倍或0.00125 mL的NOBF时未出现血细胞吸附（罗塞塔形成）现象。感染后96小时，NOBF组实时PCR获得的Ct值与初始值相同或更低（25），而阳性对照组的Ct值增加了数倍（17.84）。
试验表明NOBF可使ASFV失活。NOBF有潜力在实际应用中作为抗ASFV制剂。下一步是进行更高水平的试验以确定其功效。
