Li Zengliang, Fan Hao, Chen Wangwang, Xiao Jian, Ma Xiang, Ni Peidong, Xu Zekuan, Yang Li
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Department of General Surgery, Liyang People's Hospital, Liyang Branch Hospital of Jiangsu Province Hospital, Liyang, China.
Front Mol Biosci. 2021 Apr 15;8:655580. doi: 10.3389/fmolb.2021.655580. eCollection 2021.
MicroRNAs (miRNAs) are emerging as significant regulators of the tumorigenesis of gastric cancer (GC), and may be effective biomarkers for diagnosis, prognosis, and therapeutic targeting for GC. In this study, miR-653-5p was found to be significantly upregulated in GC tissues, serum, and cell lines and was strongly associated with poor prognosis in patients with GC. Furthermore, miR-653-5p promoted GC cell proliferation and metastasis and . Suppressor of cytokine signaling 6 (SOCS6) was directly targeted by miR-653-5p, and SOCS6 attenuated miR-653-5p-mediated GC cell growth, migration, and invasion. In addition, SOCS6-mediated inactivation of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway was also reversed by the administration of miR-653-5p. The findings from this study support a novel regulatory axis between miR-653-5p, SOCS6, and JAK2/STAT3 that may be a target for diagnosis and therapeutic intervention for GC.
微小RNA（miRNA）正成为胃癌（GC）肿瘤发生的重要调节因子，并且可能是GC诊断、预后评估及治疗靶点的有效生物标志物。在本研究中，发现miR-653-5p在GC组织、血清及细胞系中显著上调，且与GC患者的不良预后密切相关。此外，miR-653-5p促进GC细胞增殖和转移。细胞因子信号转导抑制因子6（SOCS6）是miR-653-5p的直接靶点，SOCS6可减弱miR-653-5p介导的GC细胞生长、迁移及侵袭。此外，给予miR-653-5p也可逆转SOCS6介导的Janus激酶2/信号转导及转录激活因子3（JAK2/STAT3）信号通路失活。本研究结果支持miR-653-5p、SOCS6和JAK2/STAT3之间存在一种新的调节轴，这可能是GC诊断和治疗干预的靶点。
