噬菌体 KM16 的分离、鉴定及其在生物膜中的应用。
Isolation, characterization, and application of phage KM16 against biofilm.
机构信息
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo, PR China.
Department of Clinical laboratory, The First People's Hospital of Yunnan Province, Kunming, PR China.
出版信息
Biofouling. 2021 Mar;37(3):276-288. doi: 10.1080/08927014.2021.1900130. Epub 2021 May 5.
biofilm prevention and control is of great importance. This study, investigated the use of the isolated phage KM16 belonging to the family Myoviridae in the order Caudovirales. The phage genome size was 170,126bp. Almost all phages were adsorbed to the host within 20min. KM16 had a latent period of 70min followed by a rise period of 40min. Phage KM16 had the ability to lytically infect 10 out of the 12 clinical strains of tested. Phylogenetic analysis indicated that the 16S rRNA and genes correlated with the lytic spectrum of phage KM16. The lytic spectrum of phage KM16 correlated with pili (), and pili were the recognition site for phage adsorption to the host. Phage KM16 (MOI=0.1) had a better anti-biofilm effect than kanamycin sulfate (10 ug ml) in high-concentration cultures.
生物膜的预防和控制非常重要。本研究调查了属于长尾噬菌体目的分离噬菌体 KM16 在有尾噬菌体科中的应用。噬菌体基因组大小为 170126bp。几乎所有噬菌体都在 20min 内被吸附到宿主上。KM16 的潜伏期为 70min，随后是 40min 的上升期。噬菌体 KM16 能够裂解感染测试的 12 株临床菌株中的 10 株。系统发育分析表明，16S rRNA 和 基因与噬菌体 KM16 的裂解谱相关。噬菌体 KM16 的裂解谱与菌毛（）相关，菌毛是噬菌体吸附到宿主的识别位点。噬菌体 KM16（MOI=0.1）在高浓度 培养物中比硫酸卡那霉素（10ug/ml）具有更好的抗生物膜效果。
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