Kushugulova Almagul, Löber Ulrike, Akpanova Saniya, Rysbekov Kairat, Kozhakhmetov Samat, Khassenbekova Zhanagul, Essex Morgan, Nurgozhina Ayaulym, Nurgaziyev Madiyar, Babenko Dmitriy, Markó Lajos, Forslund Sofia K
Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.
Kazakhstan Society of Human Microbiome Researchers, Nur-Sultan, Kazakhstan.
Front Cell Infect Microbiol. 2021 Apr 21;11:622735. doi: 10.3389/fcimb.2021.622735. eCollection 2021.
Probiotics and prebiotics are widely used for recovery of the human gut microbiome after antibiotic treatment. High antibiotic usage is especially common in children with developing microbiome. We hypothesized that dry Mare's milk, which is rich in biologically active substances without containing live bacteria, could be used as a prebiotic in promoting microbial diversity following antibiotic treatment in children. The present pilot study aims to determine the impacts of dry Mare's milk on the diversity of gut bacterial communities when administered during antibiotic treatment and throughout the subsequent recovery phase.
Six children aged 4 to 5 years and diagnosed with bilateral bronchopneumonia were prescribed cephalosporin antibiotics. During the 60 days of the study, three children consumed dry Mare's milk whereas the other three did not. Fecal samples were collected daily during antibiotic therapy and every 5 days after antibiotic therapy. Total DNA was isolated and taxonomic composition of gut microbiota was analyzed by 16S rRNA amplicon sequencing. To assess the immune status of the gut, stool samples were analyzed by bead-based multiplex assays.
Mare's milk treatment seems to prevent the bloom of Mollicutes, while preventing the loss of Coriobacteriales. Immunological analysis of the stool reveals an effect of Mare's milk on local immune parameters under the present conditions.
益生菌和益生元被广泛用于抗生素治疗后人体肠道微生物群的恢复。高抗生素使用率在微生物群正在发育的儿童中尤为常见。我们推测，富含生物活性物质且不含活菌的干马奶，可作为益生元用于促进儿童抗生素治疗后的微生物多样性。本初步研究旨在确定在抗生素治疗期间及随后整个恢复阶段给予干马奶对肠道细菌群落多样性的影响。
六名4至5岁被诊断为双侧支气管肺炎的儿童被开了头孢菌素类抗生素。在为期60天的研究中，三名儿童饮用干马奶，而另外三名儿童未饮用。在抗生素治疗期间每天收集粪便样本，抗生素治疗后每5天收集一次。分离总DNA，并通过16S rRNA扩增子测序分析肠道微生物群的分类组成。为评估肠道免疫状态，通过基于微珠的多重分析对粪便样本进行分析。
马奶治疗似乎可防止柔膜菌纲大量繁殖，同时防止放线菌目减少。粪便的免疫学分析显示，在当前条件下马奶对局部免疫参数有影响。
