Vergara Daniela, Feathers Cellene, Huscher Ezra L, Holmes Ben, Haas Jacob A, Kane Nolan C
Ebio, University of Colorado at Boulder, Boulder, CO, USA.
Centennial Seeds, Lafayette, CO, USA.
PeerJ. 2021 Apr 20;9:e10672. doi: 10.7717/peerj.10672. eCollection 2021.
The flowering plant , cultivated for centuries for multiple purposes, displays extensive variation in phenotypic traits in addition to its wide array of secondary metabolite production. Notably, produces two well-known secondary-metabolite cannabinoids: cannabidiolic acid (CBDA) and delta-9-tetrahydrocannabinolic acid (THCA), which are the main products sought by consumers in the medical and recreational market. has several suggested subspecies which have been shown to differ in chemistry, branching patterns, leaf morphology and other traits. In this study we obtained measurements related to phytochemistry, reproductive traits, growth architecture, and leaf morphology from 297 hybrid individuals from a cross between two diverse lineages. We explored correlations among these characteristics to inform our understanding of which traits may be causally associated. Many of the traits widely assumed to be strongly correlated did not show any relationship in this hybrid population. The current taxonomy and legal regulation within is based on phenotypic and chemical characteristics. However, we find these traits are not associated when lineages are inter-crossed, which is a common breeding practice and forms the basis of most modern marijuana and hemp germplasms. Our results suggest naming conventions based on leaf morphology do not correspond to the chemical properties in plants with hybrid ancestry. Therefore, a new system for identifying variation within is warranted that will provide reliable identifiers of the properties important for recreational and, especially, medical use.
这种开花植物已经被种植了几个世纪，用途广泛，除了能产生种类繁多的次生代谢产物外，其表型性状也有广泛的变异。值得注意的是，它能产生两种著名的次生代谢产物大麻素：大麻二酚酸（CBDA）和Δ9-四氢大麻酚酸（THCA），这是医疗和娱乐市场消费者所追求的主要产品。该植物有几个被认为的亚种，已证明它们在化学成分、分枝模式、叶片形态和其他性状上存在差异。在本研究中，我们从两个不同谱系杂交产生的297个杂种个体中获得了与植物化学、生殖性状、生长结构和叶片形态相关的测量数据。我们探索了这些特征之间的相关性，以加深我们对哪些性状可能存在因果关联的理解。在这个杂种群体中，许多被广泛认为密切相关的性状并没有表现出任何关联。目前该植物的分类学和法律法规是基于表型和化学特征的。然而，我们发现当不同谱系杂交时，这些性状并无关联，而杂交是一种常见的育种方式，也是大多数现代大麻和麻类种质的基础。我们的结果表明，基于叶片形态的命名惯例与具有杂交祖先的植物的化学性质并不对应。因此，有必要建立一个新的系统来识别该植物内部的变异，这个系统将为娱乐用途，尤其是医疗用途中重要的特性提供可靠的标识符。
