Habib Nida, Choudhry Shahnaz
Department of Biotechnology, Kinnaird College for Women, 93-Jail Road, Lahore, Pakistan.
Evid Based Complement Alternat Med. 2021 Apr 20;2021:6645680. doi: 10.1155/2021/6645680. eCollection 2021.
The medicinal importance of seeds for treating various ailments is portrayed by its traditional uses. Owing to its immense pharmacological importance, the thymoquinone phytoconstituent of can prove beneficial for the South Asian countries including Pakistan, where this seed is commonly produced and healthcare facilities are limited. In this study, the antibacterial activity of various extracts of seeds, extracted thymoquinone, and oil samples have been investigated against and using well and disc diffusion assay. The inhibition zones ranged between 7 and 44 mm against both the bacterial strains by well diffusion assay, while disc diffusion assay provided inhibition zones in the range of 7-23 mm. Commercial and local Kalonji oil samples were included in the study. Oil samples dissolved in methanol showed increased inhibition of bacteria. However, the extracted thymoquinone showed highest antibacterial activity. Medicine formulated using thymoquinone will prove to be an herbal alternate against the resistant microbiota associated with bacterial infections. Antibacterial activity against some species will help signify the effect on normal gut flora when oral therapy is followed. Trying different extraction protocols can help increase extraction efficiency. Study on extraction of thymoquinone in local produce of black seed can be fruitful for conducting the stability studies and can help to gain maximum benefits from the bioactives. The crude extracts from 10 g of these seeds were subjected to preliminary phytochemical investigation. Results showed that although methanol extract had the presence of maximum phytochemicals, hexane extract was the most potent in terms of antibacterial activity. Thymoquinone, a therapeutically important bioactive in seed, was extracted employing both solvents. TLC assay and UV spectroscopy were used for its qualitative assessment, while HPLC-UV quantification showed that 250 mg/mL of methanol extract had 368.3 g/mL thymoquinone, while its successive extraction yielded 32.94 g/mL thymoquinone.
种子在治疗各种疾病方面的药用价值通过其传统用途得以体现。由于其巨大的药理重要性，黑种草子的百里醌植物成分对包括巴基斯坦在内的南亚国家可能有益，这些国家普遍种植这种种子且医疗设施有限。在本研究中，采用打孔法和纸片扩散法研究了黑种草子的各种提取物、提取的百里醌以及油样对金黄色葡萄球菌和大肠杆菌的抗菌活性。打孔法对两种细菌菌株的抑菌圈范围在7至44毫米之间，而纸片扩散法提供的抑菌圈范围在7至23毫米之间。研究中纳入了市售和当地的黑种草子油样品。溶解在甲醇中的油样对细菌的抑制作用增强。然而，提取的百里醌显示出最高的抗菌活性。使用百里醌配制的药物将被证明是对抗与细菌感染相关的耐药微生物群的草药替代品。对某些物种的抗菌活性将有助于表明口服治疗时对正常肠道菌群的影响。尝试不同的提取方案有助于提高提取效率。对当地黑种草子产品中百里醌提取的研究对于进行稳定性研究可能富有成效，并有助于从生物活性成分中获得最大益处。对10克这些种子的粗提物进行了初步植物化学研究。结果表明，尽管甲醇提取物中植物化学成分最多，但就抗菌活性而言，己烷提取物最有效。百里醌是黑种草子中一种具有治疗重要性的生物活性成分，使用两种溶剂均可提取。采用薄层色谱法和紫外光谱法对其进行定性评估，而高效液相色谱 - 紫外定量法显示，250毫克/毫升的甲醇提取物含有368.3微克/毫升的百里醌，而其连续提取得到32.94微克/毫升的百里醌。
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