Marine College, Shandong University, Weihai, Shandong 264209, PR China.
Marine College, Shandong University, Weihai, Shandong 264209, PR China; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, PR China.
Syst Appl Microbiol. 2021 May;44(3):126209. doi: 10.1016/j.syapm.2021.126209. Epub 2021 May 5.
Two bacterial strains were isolated from a marine sediment sample taken from Jingzi Wharf, Weihai, China. These two strains were characterized at the phenotypic, chemotaxonomic, and genomic level. The two strains possessed almost identical 16S rRNA gene sequences (99.9 %). However, RAPD-PCR fingerprint patterns discriminated that they were not from one clonal origin. The average nucleotide identity (ANI) value and the digital DNA-DNA hybridization (dDDH) value between the two strains were 98.3 % and 85.4 %, respectively, suggestingthat they belonged to the same species. On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequences, the two strains belonged to the genus Salegentibacter and were closely related to S. holothuriorum KCTC 12371 (98.6 %) and S. salegens DSM 5424 (98.2-98.3 %). The ANI and dDDH clearly separated strains F63223 and F60176 from the the most related type strains with values below the thresholds for species. The genome sizes of strains F63223 and F60176 were approximate 3.89 and 3.59 Mbp, respectively. The strain F63223 had 3,335 predicted genes with DNA G + C content of 35.6 %. The major respiratory quinone was MK-6 and the major polar lipids were phosphatidylethanolamine and one unidentified lipid. According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic classification and genome analysis, the two isolates could be considered to represent a novel species of the genus Salegentibacter, for which the name Salegentibacter maritimus sp. nov., is proposed, with F63223 (=MCCC 1H00433 = KCTC 82417) as the type strain.
从中国威海荆子湾海洋沉积物样品中分离出两株细菌。这两株菌在表型、化学分类和基因组水平上进行了特征描述。这两株菌的 16S rRNA 基因序列（99.9%）几乎完全相同。然而，RAPD-PCR 指纹图谱区分表明它们不是来自一个克隆起源。两株菌的平均核苷酸同一性（ANI）值和数字 DNA-DNA 杂交（dDDH）值分别为 98.3%和 85.4%，表明它们属于同一物种。基于 16S rRNA 基因序列的系统发育分析结果，两株菌属于盐单胞菌属，与 S. holothuriorum KCTC 12371（98.6%）和 S. salegens DSM 5424（98.2-98.3%）密切相关。ANI 和 dDDH 清楚地将菌株 F63223 和 F60176 与最相关的模式菌株区分开来，其值低于种的阈值。菌株 F63223 和 F60176 的基因组大小分别约为 3.89 和 3.59 Mbp。菌株 F63223 预测有 3335 个基因，DNA G+C 含量为 35.6%。主要呼吸醌为 MK-6，主要极性脂为磷脂酰乙醇胺和一种未鉴定的脂类。根据表型、化学分类特征、系统发育分类和基因组分析结果，这两株分离物可被认为代表了盐单胞菌属的一个新种，提议将其命名为海洋盐单胞菌 Salegentibacter maritimus sp. nov.，F63223（=MCCC 1H00433=KCTC 82417）为模式菌株。
