School of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland.
Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg, Denmark.
Gut Microbes. 2021 Jan-Dec;13(1):1-19. doi: 10.1080/19490976.2021.1919464.
Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an colon model increased alpha-diversity, raised relative abundance of taxa including , and ; and introduced taxa such as spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome , paving the way for pre-clinical and human trials.
培养的肠道分离物的管理有望调节老年受试者肠道微生物群落的改变组成和功能，并促进其健康。从最初的 692 个分离物中，我们根据模拟健康受试者的肠道微生物群落，选择了 100 种肠道共生菌株（MCC100），并保留了所选物种内的菌株多样性。确定了 MCC100 对 7 种抗生素的敏感性，并对其基因组进行了毒力因子、抗微生物药物耐药性和细菌素基因的筛查。在健康和虚弱的老年人群的肠道模型中补充 MCC100 可以增加 α-多样性，提高包括 在内的分类群的相对丰度；并引入 spp. 等分类群。微生物群的变化与老年人健康相关的支链氨基酸水平升高相关。该研究表明，MCC100 联合体可以调节老年受试者的肠道微生物群落组成和相关代谢组，为临床前和人体试验铺平了道路。
