Zhang Yupeng, Xiao Fengxu, Zhang Liang, Ding Zhongyang, Shi Guiyang, Li Youran
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
Microorganisms. 2023 Sep 13;11(9):2303. doi: 10.3390/microorganisms11092303.
Catabolite control protein A (CcpA) is a critical regulator in Gram-positive bacteria that orchestrates carbon metabolism by coordinating the utilization of different carbon sources. Although it has been widely proved that CcpA helps prioritize the utilization of glucose over other carbon sources, this global regulator's precise mechanism of action remains unclear. In this study, a mutant deleted for CcpA was constructed. Cell growth, carbon utilization, metabolites and the transcription of key enzymes of the mutant strain were compared with that of the wild-type one. It was found that CcpA is involved in the regulation of glucose concentration metabolism in Bacillus. At the same time, CcpA regulates glucose metabolism by inhibiting acetic acid synthesis and pentose phosphate pathway key gene . The conversion rate of acetic acid is increased by about 3.5 times after is deleted. The present study provides a new mechanism of carbon metabolism and acetic acid balance regulated by CcpA. On the one hand, this work deepens the understanding of the regulatory function of CcpA and provides a new view on the regulation of glucose metabolism. On the other hand, it is helpful to the transformation of chassis microorganisms.
分解代谢物控制蛋白A（CcpA）是革兰氏阳性菌中的一种关键调节因子，它通过协调不同碳源的利用来调控碳代谢。尽管已有广泛证据表明CcpA有助于优先利用葡萄糖而非其他碳源，但这种全局调节因子的确切作用机制仍不清楚。在本研究中，构建了一个缺失CcpA的突变体。将突变株的细胞生长、碳利用、代谢物以及关键酶的转录与野生型进行了比较。结果发现，CcpA参与了芽孢杆菌中葡萄糖浓度代谢的调控。同时，CcpA通过抑制乙酸合成和磷酸戊糖途径关键基因来调节葡萄糖代谢。删除 后，乙酸转化率提高了约3.5倍。本研究提供了一种由CcpA调控的碳代谢和乙酸平衡的新机制。一方面，这项工作加深了对CcpA调控功能的理解，并为葡萄糖代谢调控提供了新的视角。另一方面，它有助于底盘微生物的改造。
