Jin Saiyan, Yu Changcheng, Yu Beiwei
Department of Clinical Laboratory, Hangzhou Cancer Hospital No. 34 Yanguan Lane, Shangcheng District, Hangzhou 310002, Zhejiang Province, P. R. China.
Department of Laboratory, Hangzhou Jianggan District People's Hospital No. 238-6 Jichang Road, Jianggan District, Hangzhou 321000, Zhejiang Province, P. R. China.
Am J Transl Res. 2021 Apr 15;13(4):2867-2874. eCollection 2021.
The levels of serum IL-6, IL-10, and TNF-α in patients with systemic lupus erythematosus (SLE) and their value in clinical practice were studied. A total of 68 patients with active SLE treated in our hospital between March 2015 and January 2018 were enrolled into the active SLE group, and they were divided into three groups according to the mild, moderate, and heavy active periods, and also divided into two groups according to the positive and negative anti dsDNA. A total of 60 healthy individuals in the same period were included in the control group (con group). The levels of serum IL-6, IL-10, and TNF-α in all participants were detected via an enzyme-linked immunosorbent assay (ELISA), and the correlation of these values with SLE activity was analyzed. The independent prognostic factors were analyzed through multivariate logistic regression. It was found that the levels of serum IL-6, IL-10, and TNF-α in the SLE groups were all higher than those in the control group; the levels of the inflammatory markers in the severe active SLE group were higher than those in the mild and moderate active SLE groups, and the levels in the moderate active SLE group were higher than those in the mild active SLE group. Additionally, the anti dsDNA positive group showed much higher levels of these than the anti dsDNA negative group. Pearson correlation analysis revealed a positive correlation between anti dsDNA antibody and IL-6, IL-10, and TNF-α levels. The multivariate logistic regression results, the mean course of disease and IL-10 were independent prognostic factors of SLE. The abnormal secretion of peripheral blood cytokines in SLE patients can affect the prognosis of the disease. Monitoring serum cytokines is helpful to understand the activity and prognosis of patients with lupus and guide clinical treatment.
研究了系统性红斑狼疮（SLE）患者血清白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）水平及其在临床实践中的价值。2015年3月至2018年1月在我院接受治疗的68例活动性SLE患者被纳入活动性SLE组，根据轻度、中度和重度活动期分为三组，又根据抗双链DNA阳性和阴性分为两组。同期60名健康个体被纳入对照组（con组）。通过酶联免疫吸附测定（ELISA）检测所有参与者血清IL-6、IL-10和TNF-α水平，并分析这些值与SLE活动度的相关性。通过多因素logistic回归分析独立预后因素。结果发现，SLE组血清IL-6、IL-10和TNF-α水平均高于对照组；重度活动期SLE组炎症标志物水平高于轻度和中度活动期SLE组，中度活动期SLE组高于轻度活动期SLE组。此外，抗双链DNA阳性组这些指标水平远高于抗双链DNA阴性组。Pearson相关性分析显示抗双链DNA抗体与IL-6、IL-10和TNF-α水平呈正相关。多因素logistic回归结果显示，疾病平均病程和IL-10是SLE的独立预后因素。SLE患者外周血细胞因子分泌异常可影响疾病预后。监测血清细胞因子有助于了解狼疮患者的活动度和预后，指导临床治疗。
