Callanan Mark C, Christensen Kevin D, Plummer Hillary A, Torres Johnny, Anz Adam W
Orthopedic Clinic, Shreveport, Louisiana, U.S.A.
Revere Health Orthopedics, Provo, Utah, U.S.A.
Arthrosc Sports Med Rehabil. 2021 Feb 22;3(2):e399-e410. doi: 10.1016/j.asmr.2020.10.003. eCollection 2021 Apr.
To analyze the cellular response and chemokine profiles following exercise using cooling and blood flow restriction on the Vasper system.
Healthy male patients between the ages of 20 and 39 years were recruited. Testing was performed on the Vasper system, a NuStep cross-trainer with concomitant 4-limb venous compression with proximal arm cuffs at 40 mm Hg and proximal leg cuffs at 65 mm Hg. A cooling vest and cooling mat (8.3°C) were used. A 7-minute warm-up followed by alternating 30- and 60-second sprints with 1.5 and 2 minutes of active recovery, respectively, between each sprint. Peripheral blood was drawn before exercise, immediately following exercise (T20), 10 minutes after the first post-exercise blood draw (T30), and then every 30 minutes (T60, T90, T120, T150, T180). A blood draw occurred at 24 hours' postexercise. Complete blood count, monoclonal flow cytometry for CD34+, and enzyme-linked immunosorbent assay were used to analyze the samples.
Sixteen healthy male patients (29.5 ± 4.5years, 1.78 ± 0.05m, 83.7 ± 11.4 kg) were enrolled. There was an immediate, temporary increase in white blood cell counts, marked by an increase in lymphocyte differential (38.3 ± 6.5 to 44.3 ± 9.0%, = .001), decrease in neutrophil differential (47.8 ± 6.6 to 42.0 ± 9.1%, < .001), and platelets (239.5 ± 57.2 to 268.6 ± 86.3 K⋅μL, = .01). Monocytes significantly decreased from PRE to T90 (9.8 ± 1.1 to 8.9 ± 1.1K/μL, < .001) and T120 (8.9 ± 1.1 K/μL, < .0001). There was a significant increase in CD34+ cells (3.9 ± 2.0 to 5.3 ± 2.8 cells⋅μL, < .001). No detectable differences in measured cytokine levels of interleukin (IL)-10, IL-6, granulocyte-macrophage colony-stimulating factor , IL-1ra, tumor necrosis factor-α, or IL-2 were observed.
A significant elevation of peripheral blood CD34+ and platelet levels immediately following the exercise session was observed; however, there was no effect on peripheral circulation of IL-10, IL-6, IL-1ra, tumor necrosis factor-α, or IL-2.
Exercise can be considered as a way to manipulate point-of-care blood products like platelet-rich plasma and may increase product yield.
分析在Vasper系统上使用冷却和血流限制进行运动后的细胞反应和趋化因子谱。
招募年龄在20至39岁之间的健康男性患者。在Vasper系统上进行测试，该系统是一台NuStep交叉训练机，同时通过近端手臂袖带施加40毫米汞柱的压力和近端腿部袖带施加65毫米汞柱的压力对四肢进行静脉压迫。使用了冷却背心和冷却垫（8.3°C）。先进行7分钟的热身，然后交替进行30秒和60秒的冲刺，每次冲刺之间分别有1.5分钟和2分钟的主动恢复。在运动前、运动后立即（T20）、运动后首次采血后10分钟（T30），然后每30分钟（T60、T90、T120、T150、T180）采集外周血。在运动后24小时进行采血。使用全血细胞计数、针对CD34+的单克隆流式细胞术和酶联免疫吸附测定法分析样本。
招募了16名健康男性患者（29.5±4.5岁，1.78±0.05米，83.7±11.4千克）。白细胞计数立即出现暂时升高，表现为淋巴细胞分类增加（从38.3±6.5%增至44.3±9.0%，P = 0.001）、中性粒细胞分类减少（从47.8±6.6%降至42.0±9.1%，P < 0.001）以及血小板计数增加（从239.5±57.2增至268.6±86.3千/微升，P = 0.01）。单核细胞从运动前到T90（从9.8±1.1降至8.9±1.1千/微升，P < 0.001）和T120（8.9±1.1千/微升，P < 0.0001）显著减少。CD34+细胞显著增加（从3.9±2.0增至5.3±2.8个/微升，P < 0.001）。在白细胞介素（IL）-10、IL-6、粒细胞-巨噬细胞集落刺激因子、IL-1ra、肿瘤坏死因子-α或IL-2的测量细胞因子水平上未观察到可检测到的差异。
观察到运动后外周血CD34+和血小板水平立即显著升高；然而，对IL-10、IL-6、IL-1ra、肿瘤坏死因子-α或IL-2的外周循环没有影响。
运动可被视为一种调节即时护理血液制品如富血小板血浆的方法，并且可能提高制品产量。
