Callanan Mark C, Plummer Hillary A, Chapman Garrett L, Opitz Tyler J, Rendos Nicole K, Anz Adam W
The Orthopedic Clinic, Shreveport, Louisiana, U.S.A.
Andrews Research & Education Foundation, Gulf Breeze, Florida, U.S.A.
Arthrosc Sports Med Rehabil. 2020 Dec 27;3(1):e189-e198. doi: 10.1016/j.asmr.2020.09.009. eCollection 2021 Feb.
To determine the effects of blood flow restriction (BFR) exercise on CD34 cells, platelets, white blood cells, neutrophils, lymphocytes, lactate, and glucose.
Healthy participants aged 20 to 39 years who were able to perform the exercise sessions were recruited. Participants underwent an experimental (EXP) occluded testing session and a control (CON) session using the Delfi Personalized Tourniquet System. Blood draws were performed prior to testing and immediately after the exercise session. Blood analysis consisted of a complete blood count as well as flow cytometry to measure peripheral CD34 counts as a marker for hematopoietic progenitor cells.
Fourteen men (aged 30.8 ± 3.9 years) volunteered. There was a significant increase in average CD34 counts immediately after the EXP session only (3.1 ± 1.2 cells ⋅ μL vs 5.2 ± 2.9 cells ⋅ μL, = .012). Platelet counts were significantly elevated after both sessions, with the average increase being higher after the EXP session (mean difference [MD], 34,200/μL; < .002) than after the CON session (MD, 11,600/μL; < .002). White blood cell counts significantly increased after both the EXP (8,400 ± 2,200/μL vs 6,300 ± 1,600/μL; < .001) and CON (MD, 900/μL; < .001) sessions. There was a significant increase from baseline to immediately after exercise in the average number of lymphocytes (MD, 6.3%; < .001) and, conversely, a significant decrease in the average neutrophil count (MD, 6.5%; < .001) in the EXP session only. Lactate levels significantly increased in the EXP (MD, 6.1 mmol ⋅ L; = .001) and CON (MD, 3.6 mmol ⋅ L; = .001) groups. No changes in glucose levels were observed.
Exercise with BFR causes a significant post-exercise increase in peripheral hematopoietic progenitor cells and platelets, beyond that of standard resistance training.
BFR can be considered a way to manipulate point-of-care blood products such as platelet-rich plasma to increase product yield.
确定血流限制（BFR）运动对CD34细胞、血小板、白细胞、中性粒细胞、淋巴细胞、乳酸和葡萄糖的影响。
招募年龄在20至39岁之间、能够完成运动课程的健康参与者。参与者使用德尔菲个性化止血带系统进行了一次实验性（EXP）闭塞测试课程和一次对照（CON）课程。在测试前和运动课程结束后立即进行血液采集。血液分析包括全血细胞计数以及流式细胞术，以测量外周血CD34计数作为造血祖细胞的标志物。
14名男性（年龄30.8±3.9岁）自愿参加。仅在EXP课程后，平均CD34计数立即显著增加（3.1±1.2个细胞·μL对5.2±2.9个细胞·μL，P = 0.012）。两次课程后血小板计数均显著升高，EXP课程后的平均增加量（平均差值[MD]，34,200/μL；P < 0.002）高于CON课程（MD，11,600/μL；P < 0.002）。EXP课程（8,400±2,200/μL对6,300±1,600/μL；P < 0.001）和CON课程（MD，900/μL；P < 0.001）后白细胞计数均显著增加。仅在EXP课程中，从基线到运动后立即，淋巴细胞平均数量显著增加（MD，6.3%；P < 0.001），相反，中性粒细胞平均计数显著减少（MD，6.5%；P < 0.001）。EXP组（MD，6.1 mmol·L；P = 0.001）和CON组（MD，3.6 mmol·L；P = 0.001）的乳酸水平均显著升高。未观察到葡萄糖水平的变化。
与标准抗阻训练相比，BFR运动导致运动后外周造血祖细胞和血小板显著增加。
BFR可被视为一种操纵即时护理血液制品（如富含血小板血浆）以提高产品产量的方法。
