Institute of Photonics and Electronics of the Czech Academy of Sciences, Prague, Czechia.
Sci Rep. 2021 May 25;11(1):10852. doi: 10.1038/s41598-021-89753-9.
Nowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence-BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.
如今，现代医学正在寻找新的、更温和、更有效的诊断方法。生物体的病理状态通常与活性氧（ROS）的增加密切相关。ROS 可以与生物分子发生反应，随后的反应可能导致非常低的内源性光发射（生物自动发光-BAL）。这种现象可以作为一种非侵入性的、低运营成本的工具，用于监测疾病期间的氧化应激。为了帮助理解影响 BAL 的参数，我们以酵母酿酒酵母为代表的真核生物分析了 BAL。建立了 BAL 强度与芬顿反应产生的活性氧数量之间的关系，以及自发 BAL 与细胞生长过程中选定的物理和化学参数（pH 值、氧分压和细胞浓度）之间的相关性。我们的研究结果有助于为生物医学和生物技术中的氧化过程监测提供实时、非侵入性的方法。
