Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin 300457, PR China.
Institute of Medical Support Technology, Academy of Military Science, Tianjin 300161, PR China.
Int J Biol Macromol. 2022 Sep 30;217:998-1011. doi: 10.1016/j.ijbiomac.2022.07.186. Epub 2022 Jul 28.
Development of chitosan-based hemostatic products and their application in wound healing has always been a research hotspot in the field of emergency treatment and biomedicine. In this study, the classic hemostatic chitosan and the most well-known inorganic hemostatic agent-kaolin were tried to combine to form a more excellent dressing. Together with the aid of non-toxic, harmless and good hydrophilic polyethylene oxide, chitosan/polyethylene oxide (PEO)/kaolin nanofiber membranes (CPKs) were prepared by electrospinning technology. Such membranes exhibited adjustable mechanical properties and good biocompatibility. Furthermore, a series of in vitro coagulation experiments proved that CPKs with 10 % ratio of kaolin (CPK10) has excellent hemostatic ability. Especially, in the whole blood coagulation time (WBCT) assay, the hemostatic time of CPK10 (43 ± 1.4 s) was significantly lower than that of chitosan/polyethylene oxide (CPK0) nanofiber membrane (61 ± 2.2 s) and QuikClot® Combat Gauze (55.7 ± 1.2 s). The further rat liver injury test reconfirmed that CPK10 can stop bleeding better and faster compared to other groups. In addition, CPKs could promote back wound healing in rats within 14 days without significant inflammatory response. This safe and effective hemostatic CPKs is expected to be a promising candidate hemostat in pre-hospital medical care.
壳聚糖基止血产品的开发及其在创伤愈合中的应用一直是急诊和生物医学领域的研究热点。在本研究中，尝试将经典止血壳聚糖和最知名的无机止血剂-高岭土结合起来，形成一种更优秀的敷料。在无毒、无害且亲水性良好的聚乙烯氧化物的辅助下，通过静电纺丝技术制备了壳聚糖/聚乙烯氧化物（PEO）/高岭土纳米纤维膜（CPKs）。这种膜具有可调节的机械性能和良好的生物相容性。此外，一系列体外凝血实验证明，高岭土含量为 10%的 CPKs（CPK10）具有优异的止血能力。特别是在全血凝固时间（WBCT）测定中，CPK10（43±1.4s）的止血时间明显低于壳聚糖/聚乙烯氧化物（CPK0）纳米纤维膜（61±2.2s）和 QuikClot® Combat Gauze（55.7±1.2s）。进一步的大鼠肝损伤试验再次证实，与其他组相比，CPK10 能更好更快地止血。此外，CPKs 在 14 天内可促进大鼠背部伤口愈合，且无明显炎症反应。这种安全有效的止血 CPKs 有望成为一种有前途的院前医疗用止血剂。
