Weber Sarah R, Zhao Yuanjun, Gates Christopher, Ma Jingqun, da Veiga Leprevost Felipe, Basrur Venkatesha, Nesvizhskii Alexey I, Gardner Thomas W, Sundstrom Jeffrey M
Department of Ophthalmology, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA.
Kellogg Eye Center, University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA.
J Clin Med. 2021 May 25;10(11):2309. doi: 10.3390/jcm10112309.
Vitreous fluid is becoming an increasingly popular medium for the study of retinal disease. Numerous studies have demonstrated that proteomic analysis of the vitreous from patients with proliferative diabetic retinopathy yields valuable molecular information regarding known and novel proteins and pathways involved in this disease. However, there is no standardized methodology for vitreous proteomic studies. Here, we share a suggested protocol for such studies and outline the various experimental and analytic methods that are currently available. We also review prior mass spectrometry-based proteomic studies of the vitreous from patients with proliferative diabetic retinopathy, discuss common pitfalls of these studies, and propose next steps for moving the field forward.
玻璃体液正日益成为研究视网膜疾病的热门介质。大量研究表明，对增殖性糖尿病视网膜病变患者的玻璃体液进行蛋白质组学分析，可获得有关该疾病中已知和新发现的蛋白质及相关通路的有价值分子信息。然而，玻璃体液蛋白质组学研究尚无标准化方法。在此，我们分享此类研究的建议方案，并概述目前可用的各种实验和分析方法。我们还回顾了先前基于质谱的增殖性糖尿病视网膜病变患者玻璃体液蛋白质组学研究，讨论这些研究的常见陷阱，并提出推动该领域发展的下一步措施。
