Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
J Agric Food Chem. 2021 Jun 16;69(23):6710-6719. doi: 10.1021/acs.jafc.1c02241. Epub 2021 Jun 3.
is one of the most recognized ginseng species. In this study, lipidome and metabolome extraction methods for were optimized with methanol/methyl--butyl ether/water (0.3 mg/1 μL/6 μL/8 μL). A total of 497 metabolites were identified, including 365 lipids and 76 ginsenosides. Comprehensive lipidome profiling was first performed for , in which 32.6% glycerophospholipids, 39.5% glycerolipids, 9.3% sphingolipids, 3.3% sterol lipids, and 15.3% fatty acyls were identified. Orthogonal partial least squares discrimination analysis (OPLS-DA) showed obvious metabolomic differences in two growing regions of China. In the northern growing region, the ratio of bilayer- to nonbilayer-forming membrane lipids (PCs/PEs, DGDGs/MGDGs), the degree of unsaturation of acyl chains in galactolipids, and the content of membrane glycerophospholipids were increased. In the eastern growing region, the synthesis of storage lipids, ceramides, and fatty acyls was increased, and secondary metabolism was enhanced with 24 differential ginsenosides found. The investigation deepens the understanding of metabolic regulation mechanisms of .
是最受认可的人参品种之一。在这项研究中，采用甲醇/甲基叔丁基醚/水（0.3 mg/1 μL/6 μL/8 μL）优化了 的脂质组学和代谢组学提取方法。共鉴定出 497 种代谢物，包括 365 种脂质和 76 种人参皂苷。首次对 进行了全面的脂质组学分析，其中 32.6%的甘油磷脂、39.5%的甘油酯、9.3%的鞘脂、3.3%的甾醇脂和 15.3%的脂肪酸。正交偏最小二乘判别分析（OPLS-DA）显示中国两个不同生长地区的代谢组学存在明显差异。在北方生长地区，双层膜形成与非双层膜形成的膜脂（PCs/PEs、DGDGs/MGDGs）的比例、半乳糖脂中酰链的不饱和程度以及膜甘油磷脂的含量增加。在东部生长地区，储存脂质、神经酰胺和脂肪酸的合成增加，并且发现了 24 种差异人参皂苷，增强了次级代谢。该研究加深了对 的代谢调控机制的理解。
