Medical Examination Center, The Third Hospital of Jinan, Jinan, 250132, China.
Department of Obstetrics, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250013, Shandong, China.
World J Surg Oncol. 2021 Jun 5;19(1):165. doi: 10.1186/s12957-021-02274-7.
X inactivation-specific transcript (XIST) is the long non-coding RNA (lncRNA) related to cancer, which is involved in the development and progression of various types of tumor. However, up to now, the exact role and molecular mechanism of XIST in the progression of ovarian cancer are not clear. We studied the function of XIST in ovarian cancer cells and clinical tumor specimens.
RT-qPCR was performed to detect the expression levels of miR-335 and BCL2L2 in ovarian cancer cells and tissues. MTT and transwell assays were carried out to detect cell proliferation, migration, and invasion abilities. Western blot was performed to analyze the expression level of BCL2L2. The interaction between miR-335 and XIST/BCL2L2 was confirmed using a luciferase reporter assay.
The inhibition of XIST can inhibit the proliferation invasion and migration of human ovarian cancer cells. In addition, the miR-335/BCL2L2 axis was involved in the functions of XIST in ovarian cancer cells. These results suggested that XIST could regulate tumor proliferation and invasion and migration via modulating miR-335/BCL2L2.
XIST might be a carcinogenic lncRNA in ovarian cancer by regulating miR-335, and it can serve as a therapeutic target in human ovarian cancer.
X 失活特异性转录物（XIST）是一种与癌症相关的长链非编码 RNA（lncRNA），参与各种类型肿瘤的发生和发展。然而，到目前为止，XIST 在卵巢癌进展中的确切作用和分子机制尚不清楚。我们研究了 XIST 在卵巢癌细胞和临床肿瘤标本中的功能。
采用 RT-qPCR 检测卵巢癌细胞和组织中 miR-335 和 BCL2L2 的表达水平。采用 MTT 和 Transwell 实验检测细胞增殖、迁移和侵袭能力。采用 Western blot 分析 BCL2L2 的表达水平。采用荧光素酶报告实验证实 miR-335 与 XIST/BCL2L2 的相互作用。
抑制 XIST 可抑制人卵巢癌细胞的增殖、侵袭和迁移。此外，miR-335/BCL2L2 轴参与了 XIST 在卵巢癌细胞中的功能。这些结果表明，XIST 可能通过调节 miR-335/BCL2L2 来调节肿瘤的增殖、侵袭和迁移。
XIST 可能通过调节 miR-335 在卵巢癌中发挥致癌 lncRNA 的作用，可作为人类卵巢癌的治疗靶点。
