Department of Biological Sciences, National University of Singapore, Singapore, 117557, Singapore.
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.
Nat Commun. 2021 Jun 7;12(1):3403. doi: 10.1038/s41467-021-23728-2.
Developmental outcomes are shaped by the interplay between intrinsic and external factors. The production of stomata-essential pores for gas exchange in plants-is extremely plastic and offers an excellent system to study this interplay at the cell lineage level. For plants, light is a key external cue, and it promotes stomatal development and the accumulation of the master stomatal regulator SPEECHLESS (SPCH). However, how light signals are relayed to influence SPCH remains unknown. Here, we show that the light-regulated transcription factor ELONGATED HYPOCOTYL 5 (HY5), a critical regulator for photomorphogenic growth, is present in inner mesophyll cells and directly binds and activates STOMAGEN. STOMAGEN, the mesophyll-derived secreted peptide, in turn stabilizes SPCH in the epidermis, leading to enhanced stomatal production. Our work identifies a molecular link between light signaling and stomatal development that spans two tissue layers and highlights how an environmental signaling factor may coordinate growth across tissue types.
发育结果是由内在和外在因素共同作用决定的。气孔——植物进行气体交换的必要孔道——的产生具有极强的可塑性，为在细胞谱系水平上研究这种相互作用提供了一个极好的系统。对于植物来说，光是一个关键的外在信号，它能促进气孔的发育和主调控因子沉默突变体（SPCH）的积累。然而，光信号是如何被传递来影响 SPCH 的仍然未知。在这里，我们表明，光调控转录因子长日素 5（HY5），一个光形态建成生长的关键调控因子，存在于内叶肉细胞中，并直接结合并激活气孔生成基因（STOMAGEN）。反过来，叶肉衍生的分泌肽 STOMAGEN 稳定表皮中的 SPCH，导致气孔产生增加。我们的工作确定了光信号和气孔发育之间的分子联系，该联系跨越两个组织层，并强调了环境信号因子如何协调不同组织类型的生长。
