School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, 453003, China.
Biochem Genet. 2022 Feb;60(1):127-152. doi: 10.1007/s10528-021-10093-4. Epub 2021 Jun 12.
miR160 plays a crucial role in various biological processes by regulating their target gene auxin response factor (ARF) in plants. However, little is known about miR160 and ARF in cucumber fruit expansion. Here, 4 Csa-MIR160 family members and 17 CsARFs were identified through a genome-wide search. Csa-miR160 showed a closer relationship with those in melon. Phylogenetic analysis revealed that CsARFs were divided into four classes and most of CsARFs presented a closer evolutionary relationship with those from tomato. Putative cis-elements analysis predicted that Csa-MIR160 and CsARFs were involved in light, phytohormone and stress response, which proved that they might take part in light, phytohormone and stress signaling pathway by the miR160-ARF module. In addition, CsARF5, CsARF11, CsARF13 and CsARF14 were predicted as the target genes of Csa-miR160. qRT-PCR revealed that Csa-miR160 and their target gene CsARFs were differentially expressed in differential cucumber tissues and developmental stages. Csa-miR160d was only expressed in the expanded cucumber fruit. CsARF5, CsARF11 and CsARF13 exhibited the lower expression in the expanded fruit than those in the ovary, while, CsARF14 showed the reverse trend. Our results suggested that Csa-miR160d might play a crucial role in cucumber fruit expansion by negatively targeting CsARF5, CsARF11 and CsARF13. This is the first genome-wide analysis of miR160 in cucumber. These findings provide useful information and resources for further studying the role of miR160 and ARF in cucumber fruit expansion.
miR160 通过调节植物中的生长素响应因子 (ARF) 靶基因在各种生物过程中发挥关键作用。然而，关于黄瓜果实扩张过程中的 miR160 和 ARF 知之甚少。本研究通过全基因组搜索鉴定了 4 个 Csa-MIR160 家族成员和 17 个 CsARFs。Csa-miR160 与甜瓜中的 miR160 关系更为密切。系统发育分析表明，CsARFs 分为 4 类，大多数 CsARFs 与番茄中的 ARFs 进化关系更为密切。顺式作用元件分析预测，Csa-MIR160 和 CsARFs 参与光、植物激素和应激反应，这证明它们可能通过 miR160-ARF 模块参与光、植物激素和应激信号通路。此外，预测 CsARF5、CsARF11、CsARF13 和 CsARF14 是 Csa-miR160 的靶基因。qRT-PCR 显示 Csa-miR160 及其靶基因 CsARFs 在不同黄瓜组织和发育阶段的表达存在差异。Csa-miR160d 仅在扩展的黄瓜果实中表达。CsARF5、CsARF11 和 CsARF13 在扩展果实中的表达低于在卵巢中的表达，而 CsARF14 则表现出相反的趋势。研究结果表明，Csa-miR160d 通过负向靶向 CsARF5、CsARF11 和 CsARF13 在黄瓜果实扩张中发挥关键作用。这是首次对黄瓜中的 miR160 进行全基因组分析。这些发现为进一步研究 miR160 和 ARF 在黄瓜果实扩张中的作用提供了有用的信息和资源。
