Mantovani R, Malgaretti N, Giglioni B, Comi P, Cappellini N, Nicolis S, Ottolenghi S
Centro sullo Studio della Patologia Cellulare, CNR, Milano, Italy.
Nucleic Acids Res. 1987 Nov 25;15(22):9349-64. doi: 10.1093/nar/15.22.9349.
Using the electrophoretic mobility shift assay and the footprinting technique, we studied the binding of nuclear proteins from erythroid and non erythroid human cells to the promoter region of the human gamma-globin gene. Two regions (A and B) of the promoter are bound by proteins present in uninduced K562 cells, but not in induced K562 cells nor in fetal liver erythroblasts; a protein binding to region A is also present in a variety of lymphoid and myeloid cells. Region B is centered on an octamer sequence identical to that present in immunoglobulin promoter and enhancers and other eukaryotic promoters; a B region binding protein common to K562 and other cells efficiently binds the octamer containing region of the histone H2B gene, while different B region proteins are more specific for uninduced K562 cells and the gamma-globin octamer containing fragment. The possible role of these nuclear proteins in gamma-globin gene regulation and/or cell differentiation is discussed.
我们运用电泳迁移率变动分析和足迹技术，研究了来自人类红系和非红系细胞的核蛋白与人γ-珠蛋白基因启动子区域的结合情况。该启动子的两个区域（A和B）可被未诱导的K562细胞中的蛋白质结合，但在诱导的K562细胞和胎儿肝脏成红细胞中则不然；与区域A结合的一种蛋白质也存在于多种淋巴细胞和髓细胞中。区域B以一个与免疫球蛋白启动子、增强子及其他真核启动子中存在的八聚体序列相同的序列为中心；K562细胞和其他细胞共有的一种B区域结合蛋白能有效结合组蛋白H2B基因的含八聚体区域，而不同的B区域蛋白对未诱导的K562细胞和含γ-珠蛋白八聚体的片段更具特异性。本文讨论了这些核蛋白在γ-珠蛋白基因调控和/或细胞分化中的可能作用。
