Li Aimin, Liu Jia, Ding Fangli, Wu Xiaolei, Pan Cong, Wang Qing, Gao Ming, Duan Shenglin, Han Xiaofeng, Xia Kai, Liu Shiwei, Wu Yimin, Zhou Zhiqiao, Zhang Xi, Gao Xiao-Dong
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology Jiangnan University Wuxi China.
New Era Health Industry (Group) CO., Ltd. Beijing China.
Food Sci Nutr. 2021 Mar 29;9(6):2894-2907. doi: 10.1002/fsn3.2246. eCollection 2021 Jun.
This work focused on the separation of the active ingredients of maca ( Walpers) and evaluated the antioxidative capability of these components with effects on improving glucose and lipid metabolism in insulin-resistant HepG2 cells. DPPH free radical scavenging and reducing power assays were used to evaluate the antioxidant activity of maca extracts. An insulin-resistant HepG2 cell model induced by glucose, fructose, oleic acid, and palmitic acid was adopted to investigate the effects of maca extracts on regulating glucose and lipid metabolism in this study. LC-MS/MS was then used for determination of the maca n-butanol (NBT) subfraction. The results showed that maca ethanol extract and subfractions of this extract exhibited certain antioxidant capacity. Furthermore, the NBT subfraction reversed the disorders in glucose and lipid metabolism in insulin-resistant HepG2 cells and significantly increased the mRNA expression of phosphoinositide 3-kinases () and in insulin-resistant HepG2 cells in a dose-dependent manner. In addition, the LC-MS/MS results showed that the NBT subfraction contained many active ingredients. Overall, this study suggests that the NBT subfraction of the ethanol extract rich in glucosinolates modulates insulin resistance via / activation in insulin-resistant HepG2 cells and might exert potentially beneficial effects in improving or treating glucose and lipid metabolic disorders.
本研究聚焦于玛咖（Lepidium meyenii Walpers）活性成分的分离，并评估了这些成分的抗氧化能力及其对改善胰岛素抵抗的HepG2细胞葡萄糖和脂质代谢的影响。采用DPPH自由基清除和还原能力测定法评估玛咖提取物的抗氧化活性。本研究采用由葡萄糖、果糖、油酸和棕榈酸诱导的胰岛素抵抗HepG2细胞模型，以研究玛咖提取物对调节葡萄糖和脂质代谢的影响。随后使用液相色谱-串联质谱法（LC-MS/MS）测定玛咖正丁醇（NBT）亚组分。结果表明，玛咖乙醇提取物及其亚组分具有一定的抗氧化能力。此外，NBT亚组分逆转了胰岛素抵抗HepG2细胞中葡萄糖和脂质代谢的紊乱，并以剂量依赖性方式显著增加了胰岛素抵抗HepG2细胞中磷酸肌醇3激酶（PI3K）和Akt的mRNA表达。此外，LC-MS/MS结果表明，NBT亚组分含有许多活性成分。总体而言，本研究表明，富含芥子油苷的乙醇提取物的NBT亚组分通过在胰岛素抵抗的HepG2细胞中激活PI3K/Akt来调节胰岛素抵抗，并可能在改善或治疗葡萄糖和脂质代谢紊乱方面发挥潜在的有益作用。
