Wee Yinshen, Yang Chieh-Hsiang, Chen Shau-Kwaun, Yen Yu-Chun, Wang Ching-Shuen
Department of Pathology University of Utah Salt Lake City UT USA.
Huntsman Cancer Institute University of Utah Salt Lake City UT USA.
Food Sci Nutr. 2021 Apr 10;9(6):3240-3249. doi: 10.1002/fsn3.2286. eCollection 2021 Jun.
Inositol hexaphosphate (IP6) is a dietary compound commonly obtained from corn, rice, etc. Although we may consume significant amount of IP6 daily, it is unclear whether this diet will impact macrophages' fate and function. Therefore, we characterized the underlying relationship between IP6 and macrophage polarization in this study. We specifically examined the signature gene expression profiles associated with pro- and anti-inflammatory responses, and resolution of inflammation pathways in macrophages under the influence of IP6. Interestingly, our data suggested that IP6 polarizes bone marrow-derived macrophages (BMDM) into an M2a-like subtype. Our results also demonstrated that IP6 reduces lipopolysaccharide-induced apoptosis and pro-inflammatory responses in macrophages. In contrast, the expression levels of genes related to anti-inflammatory responses and resolution of inflammation pathways are upregulated. Our findings collectively demonstrated that IP6 has profound modulation effects on macrophages, which warrant further research on the therapeutic benefits of IP6 for inflammatory diseases.
肌醇六磷酸（IP6）是一种常见于玉米、大米等食物中的膳食化合物。尽管我们每天可能摄入大量的IP6，但尚不清楚这种饮食是否会影响巨噬细胞的命运和功能。因此，在本研究中我们对IP6与巨噬细胞极化之间的潜在关系进行了表征。我们特别研究了与促炎和抗炎反应以及IP6影响下巨噬细胞中炎症消退途径相关的标志性基因表达谱。有趣的是，我们的数据表明IP6将骨髓来源的巨噬细胞（BMDM）极化为类似M2a的亚型。我们的结果还表明，IP6可减少脂多糖诱导的巨噬细胞凋亡和促炎反应。相反，与抗炎反应和炎症消退途径相关的基因表达水平上调。我们的研究结果共同表明，IP6对巨噬细胞具有深远的调节作用，这值得进一步研究IP6对炎症性疾病的治疗益处。
