Department of Robotics Engineering, DGIST-ETH Microrobotics Research Center, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 42988, Republic of Korea.
Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, The Catholic University, Seoul, 06591, Republic of Korea.
Adv Healthc Mater. 2021 Oct;10(19):e2100801. doi: 10.1002/adhm.202100801. Epub 2021 Jun 23.
Targeted stem cell delivery with microrobots has emerged as a potential alternative therapeutic strategy in regenerative medicine, and intranasal administration is an effective approach for minimally invasive delivery of therapeutic agents into the brain. In this study, a magnetically powered stem cell-based microrobot ("Cellbot") is used for minimally invasive targeted stem cell delivery to the brain through the intranasal passage. The Cellbot is developed by internalizing superparamagnetic iron oxide nanoparticles (SPIONs) into human nasal turbinate stem cells. The SPIONs have no influence on hNTSC characteristics, including morphology, cell viability, and neuronal differentiation. The Cellbots are capable of proliferation and differentiation into neurons, neural precursor cells, and neurogliocytes. The Cellbots in the microfluidic channel can be reliably manipulated by an external magnetic field for orientation and position control. Using an ex vivo model based on brain organoids, it is determined that the Cellbots can be transplanted into brain tissue. Using a murine model, it is demonstrated that the Cellbots can be intranasally administered and magnetically guided to the target tissue in vivo. This approach has the potential to effectively treat central nervous system disorders in a minimally invasive manner.
磁性控制的基于干细胞的微机器人（“Cellbot”）可用于通过经鼻腔途径进行微创靶向干细胞递送到大脑，作为再生医学中一种有前途的替代治疗策略，它已经出现。本研究中，Cellbot 是通过将超顺磁氧化铁纳米颗粒（SPIONs）内化到人鼻甲骨干细胞中而开发的。SPIONs 对 hNTSC 的特征没有影响，包括形态、细胞活力和神经元分化。Cellbots 能够增殖并分化为神经元、神经前体细胞和神经胶质细胞。微流道中的 Cellbots 可以通过外部磁场进行可靠地操纵，以实现定向和位置控制。使用基于脑类器官的离体模型，确定 Cellbots 可以被移植到脑组织中。使用小鼠模型，证明 Cellbots 可以经鼻腔给予，并在体内通过磁场引导到靶组织。这种方法有可能以微创的方式有效地治疗中枢神经系统疾病。
