Gundersen Rebekah Anamarie, Chu Tianyuan, Abolfathi Kiana, Dogan Serap Gokcen, Blair Phoebe Elizabeth, Nago Nyasha, Hamblin Michael, Brooke Greg Nicholas, Zwacka Ralf Michael, Hoshiar Ali Kafash, Mohr Andrea
School of Life Sciences, Protein Structure and Mechanism of Disease Group, Cancer and Stem Cell Biology Laboratory, University of Essex, Colchester CO4 3SQ, UK.
School of Computer Science and Electronic Engineering, University of Essex, Colchester CO4 3SQ, UK.
Cancer Nanotechnol. 2023 May 19;14:54. doi: 10.1186/s12645-023-00203-9.
Combining the power of magnetic guidance and the biological activities of stem cells transformed into biohybrid microrobots holds great promise for the treatment of several diseases including cancer.
We found that human MSCs can be readily loaded with magnetic particles and that the resulting biohybrid microrobots could be guided by a rotating magnetic field. Rotating magnetic fields have the potential to be applied in the human setting and steer therapeutic stem cells to the desired sites of action in the body. We could demonstrate that the required loading of magnetic particles into stem cells is compatible with their biological activities. We examined this issue with a particular focus on the expression and functionality of therapeutic genes inside of human MSC-based biohybrid microrobots. The loading with magnetic particles did not cause a loss of viability or apoptosis in the human MSCs nor did it impact on the therapeutic gene expression from the cells. Furthermore, the therapeutic effect of the gene products was not affected, and the cells also did not lose their migration potential.
These results demonstrate that the fabrication of guidable MSC-based biohybrid microrobots is compatible with their biological and therapeutic functions. Thus, MSC-based biohybrid microrobots represent a novel way of delivering gene therapies to tumours as well as in the context of other diseases.
将磁导向的力量与转化为生物杂交微型机器人的干细胞的生物活性相结合，在治疗包括癌症在内的多种疾病方面具有巨大潜力。
我们发现人类间充质干细胞可以很容易地负载磁性颗粒，并且由此产生的生物杂交微型机器人可以由旋转磁场引导。旋转磁场有潜力应用于人体，并将治疗性干细胞引导至体内所需的作用部位。我们能够证明将所需的磁性颗粒负载到干细胞中与其生物活性是相容的。我们特别关注基于人类间充质干细胞的生物杂交微型机器人内部治疗基因的表达和功能，对这个问题进行了研究。负载磁性颗粒不会导致人类间充质干细胞丧失活力或发生凋亡，也不会影响细胞的治疗基因表达。此外，基因产物的治疗效果不受影响，细胞也没有丧失其迁移潜力。
这些结果表明，基于间充质干细胞的可引导生物杂交微型机器人的制造与其生物学和治疗功能是相容的。因此，基于间充质干细胞的生物杂交微型机器人代表了一种向肿瘤以及在其他疾病背景下递送基因疗法的新方法。
