Lee Jongsung, Nho Youn Hwa, Yun Seok Kyun, Hwang Young Sun
Department of Genetic Engineering, Sungkyunkwan University, Suwon, 164-19, Republic of Korea.
COSMAX R&I Center, COSMAX Inc., Seongnam, 134-86, Republic of Korea.
BMC Complement Altern Med. 2017 Feb 16;17(1):113. doi: 10.1186/s12906-017-1619-1.
The fruit of the Terminalia chebula tree has been widely used for the treatment of various disorders. Its anti-diabetic, anti-mutagenic, anti-oxidant, anti-bacterial, anti-fungal, and anti-viral effects have been studied. Dental plaque bacteria (DPB) are intimately associated with gingivitis and periodontitis. In the quest for materials that will prove useful in the treatment and prevention of periodontal disease, we investigated the preventive effects of an ethanol extract of Terminalia chebula (EETC) on DPB-induced inflammation and bone resorption.
The anti-bacterial effect of EETC was analyzed using the disc diffusion method. The anti-inflammatory effect of EETC was determined by molecular biological analysis of the DPB-mediated culture cells. Prevention of osteoclastic bone resorption by EETC was explored using osteoclast formation and pit formation assays.
EETC suppressed the growth of oral bacteria and reduced the induction of inflammatory cytokines and proteases, abolishing the expression of PGE2 and COX-2 and inhibiting matrix damage. By stimulating the DPB-derived lipopolysaccharides, EETC inhibited both osteoclast formation in osteoclast precursors and RANKL expression in osteoblasts, thereby contributing to the prevention of bone resorption.
EETC may be a beneficial supplement to help prevent DPB-mediated periodontal disease.
诃子果实已被广泛用于治疗各种疾病。其抗糖尿病、抗诱变、抗氧化、抗菌、抗真菌和抗病毒作用已得到研究。牙菌斑细菌（DPB）与牙龈炎和牙周炎密切相关。在寻找对牙周疾病治疗和预防有用的材料过程中，我们研究了诃子乙醇提取物（EETC）对DPB诱导的炎症和骨吸收的预防作用。
采用纸片扩散法分析EETC的抗菌作用。通过对DPB介导的培养细胞进行分子生物学分析来确定EETC的抗炎作用。使用破骨细胞形成和凹坑形成试验探究EETC对破骨细胞性骨吸收的预防作用。
EETC抑制口腔细菌生长，减少炎症细胞因子和蛋白酶的诱导，消除PGE2和COX-2的表达并抑制基质损伤。通过刺激DPB衍生的脂多糖，EETC抑制破骨细胞前体中的破骨细胞形成和成骨细胞中的RANKL表达，从而有助于预防骨吸收。
EETC可能是预防DPB介导的牙周疾病的有益补充剂。
