Climova Alina, Ibrahim Monica N G, Salamahina Alisa, Savin Artemii M, Dukhinova Marina S, Barakova Nadezhda V, Krivoshapkina Elena F
SCAMT Institute, ITMO University, Lomonosova Street 9, Saint Petersburg, 191002 Russia.
Department of Food Biotechnology for Plant Origin Products, Faculty of Food Biotechnologies and Engineering, ITMO University, Lomonosova Street 9, Saint Petersburg, 191002 Russia.
J Food Sci Technol. 2021 Jul;58(7):2641-2650. doi: 10.1007/s13197-020-04770-2. Epub 2020 Sep 6.
The cell walls of cereals are rich sources of polysaccharide β-glucan. In this study, the β-glucan was extracted from oat bran using the hot-water extraction method and dried in a pure powder form. The concentration of the β-glucan in the extract was determined using the l-cysteine sulfuric acid method. The results showed that the yield of β-glucan using the hot-water extraction method is the highest compared to its yield achieved by enzymatic, acid, and alkaline methods. In this paper, the usage of the β-glucan as a coating material for a water-insoluble carotenoid is considered. This study demonstrates for the first time the encapsulation of β-carotene with modified octanoic acid β-glucan. It implements to obtain a stable encapsulated polysaccharide-carotenoid system, which has been studied by a set of physicochemical methods and a cytotoxic analysis was performed on the HCT-116 cell line. The SEM image of the resulting encapsulated system is perfectly correlated with the DLS data, which has determined the size of MG capsules at 200 nm. The cytotoxic analysis demonstrates that the cell viability was more than 70%, which indicates its potential using in the food industry.
谷物的细胞壁是多糖β-葡聚糖的丰富来源。在本研究中，采用热水提取法从燕麦麸中提取β-葡聚糖，并干燥成纯粉末形式。使用L-半胱氨酸硫酸法测定提取物中β-葡聚糖的浓度。结果表明，与酶法、酸法和碱法相比，热水提取法的β-葡聚糖产率最高。本文考虑了β-葡聚糖作为水不溶性类胡萝卜素包衣材料的用途。本研究首次证明了用改性辛酸β-葡聚糖包封β-胡萝卜素。它用于获得稳定的包封多糖-类胡萝卜素体系，该体系已通过一系列物理化学方法进行了研究，并对HCT-116细胞系进行了细胞毒性分析。所得包封体系的扫描电子显微镜图像与动态光散射数据完美相关，动态光散射数据确定了MG胶囊的尺寸为200纳米。细胞毒性分析表明细胞活力超过70%，这表明其在食品工业中的应用潜力。
