Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Toxins (Basel). 2021 Jun 28;13(7):446. doi: 10.3390/toxins13070446.
Zearalenone (ZEN) and its metabolites, alpha-zearalenol (α-ZEL) and beta-zearalenol (-ZEL), are ubiquitous in plant materials used as feed components in dairy cattle diets. The aim of this study was to confirm the occurrence of ZEN and its selected metabolites in blood samples collected from different sites in the hepatic portal system (posthepatic-external jugular vein EJV; prehepatic-abdominal subcutaneous vein ASV and median caudal vein MCV) of dairy cows diagnosed with mastitis, ovarian cysts and pyometra. The presence of mycotoxins in the blood plasma was determined with the use of combined separation methods involving immunoaffinity columns, a liquid chromatography system and a mass spectrometry system. The parent compound was detected in all samples collected from diseased cows, whereas α-ZEL and β-ZEL were not identified in any samples, or their concentrations were below the limit of detection (LOD). Zearalenone levels were highest in cows with pyometra, where the percentage share of average ZEN concentrations reached 44%. Blood sampling sites were arranged in the following ascending order based on ZEN concentrations: EJV (10.53 pg/mL, 44.07% of the samples collected from this site), ASV (14.20 pg/mL, 49.59% of the samples) and MCV (26.67 pg/mL, 67.35% of the samples). The results of the study indicate that blood samples for toxicological analyses should be collected from the MCV (prehepatic vessel) of clinically healthy cows and/or cows with subclinical ZEN mycotoxicosis. This sampling site increases the probability of correct diagnosis of subclinical ZEN mycotoxicosis.
玉米赤霉烯酮（ZEN）及其代谢物α-玉米赤霉烯醇（α-ZEL）和β-玉米赤霉烯醇（β-ZEL）广泛存在于用作奶牛饲料成分的植物材料中。本研究旨在确认在患有乳腺炎、卵巢囊肿和子宫积脓的奶牛的肝门静脉系统不同部位（肝后-颈外静脉 EJV；肝前-腹壁皮下静脉 ASV 和中尾静脉 MCV）采集的血液样本中是否存在 ZEN 和其选择的代谢物。使用包括免疫亲和柱、液相色谱系统和质谱系统的组合分离方法，确定了血浆中霉菌毒素的存在。在从患病奶牛采集的所有样本中均检测到母体化合物，而在任何样本中均未检测到α-ZEL 和β-ZEL，或其浓度低于检测限（LOD）。在患有子宫积脓的奶牛中，检测到最高水平的 ZEN，其中平均 ZEN 浓度的百分比达到 44%。基于 ZEN 浓度，对采血部位进行了以下升序排列：EJV（10.53 pg/mL，来自该部位的 44.07%的样本）、ASV（14.20 pg/mL，来自该部位的 49.59%的样本）和 MCV（26.67 pg/mL，来自该部位的 67.35%的样本）。研究结果表明，应从 MCV（肝前血管）采集用于毒理学分析的血液样本，来自来自患有临床健康奶牛和/或亚临床 ZEN 霉菌毒素病的奶牛。该采样部位增加了正确诊断亚临床 ZEN 霉菌毒素病的可能性。
