Guo Yunqing, Yin Mengyu, Zhang Tengfei, Zhang Wenting, Lu Qin, Hu Qiao, Zhai Xinguo, Luo Qingping
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, PR China.
Poult Sci. 2025 Jul 3;104(10):105514. doi: 10.1016/j.psj.2025.105514.
Infectious coryza (IC), caused by Avibacterium paragallinarum, is a major respiratory disease in chickens. It causes growth retardation in broilers and reduced egg production in layers, ultimately leading to huge economic losses to the poultry industry. In this study, we aimed to investigate the prevalence and genetic characteristics of 12 A. paragallinarum strains isolated from Hubei Province in 2022-2023. Whole-genome sequencing and average nucleotide identity analysis demonstrated close phylogenetic relationships between the isolates and domestic reference strains. Serotyping using the hemagglutination inhibition test identified six strains as serovar A, four as serovar B, and two as serovar C. All isolates were nicotinamide adenine dinucleotide-dependent. Antimicrobial susceptibility testing revealed that all isolates exhibited resistance to tetracycline and lincomycin and sensitivity to levofloxacin. The isolates demonstrated varying degrees of resistance to other antibiotics, including erythromycin, doxycycline, cefotaxime, florfenicol, polymyxin B, spectinomycin, and amikacin. Pathogenicity analysis revealed a wide range of virulence among the isolates, from completely virulent (100 %) to avirulent (0 %). Additionally, 14 categories of antibiotic-resistance genes, including peptide antibiotic-resistance genes, and 12 categories of virulence genes, such as adherence factor genes, were predicted using the CARD and VFDB databases. Notably, no genetic divergence was detected in resistance or virulence determinants among the 12 isolates. Overall, our study provides valuable data for the clinical management of A. paragallinarum and offers insights into its pathogenicity and antibiotic-resistance mechanisms for future studies.
由副鸡禽杆菌引起的传染性鼻炎（IC）是鸡的一种主要呼吸道疾病。它导致肉鸡生长发育迟缓，蛋鸡产蛋量下降，最终给家禽业造成巨大经济损失。在本研究中，我们旨在调查2022 - 2023年从湖北省分离出的12株副鸡禽杆菌菌株的流行情况和遗传特征。全基因组测序和平均核苷酸同一性分析表明，这些分离株与国内参考菌株之间存在密切的系统发育关系。使用血凝抑制试验进行血清型鉴定，确定6株为A血清型，4株为B血清型，2株为C血清型。所有分离株均依赖烟酰胺腺嘌呤二核苷酸。药敏试验表明，所有分离株对四环素和林可霉素耐药，对左氧氟沙星敏感。这些分离株对其他抗生素，包括红霉素、强力霉素、头孢噻肟、氟苯尼考、多粘菌素B、壮观霉素和阿米卡星，表现出不同程度的耐药性。致病性分析显示，这些分离株的毒力范围很广，从完全有毒力（100%）到无毒力（0%）。此外，使用CARD和VFDB数据库预测了14类抗生素抗性基因，包括肽类抗生素抗性基因，以及12类毒力基因，如黏附因子基因。值得注意的是，在12株分离株中未检测到耐药或毒力决定因素的遗传差异。总体而言，我们的研究为副鸡禽杆菌的临床管理提供了有价值的数据，并为其致病性和抗生素耐药机制的未来研究提供了见解。
