Instituto de Biología Integrativa de Sistemas, Universitat de València-CSIC, Paterna, Spain.
Instituto de Investigación Sanitaria La Fe, Hospital Universitari I Politècnic La Fe, Valencia, Spain.
Eur J Clin Microbiol Infect Dis. 2021 Dec;40(12):2665-2667. doi: 10.1007/s10096-021-04304-4. Epub 2021 Jul 9.
The SARS-CoV-2 can be excreted in feces and can reach sewage systems. Determining the presence of infective viral particles in feces and sewage is necessary to take adequate control measures and to elucidate new routes of transmission. Here, we have developed a sample concentration methodology that allows us to maintain viral infectivity. Feces of COVID-19 patients and wastewater samples have been analyzed both by molecular methods and cell culture. Our results show no evidence of infective viral particles, suggesting that fecal-oral transmission is not a primary route. However, larger-scale efforts are needed, especially with the emergence of new viral variants.
SARS-CoV-2 可以在粪便中排出，并可能进入污水系统。为了采取充分的控制措施并阐明新的传播途径，有必要确定粪便和污水中存在具有感染性的病毒颗粒。在此，我们开发了一种可以保持病毒感染力的样本浓缩方法。我们用分子方法和细胞培养分析了 COVID-19 患者的粪便和污水样本。我们的结果没有发现具有感染性的病毒颗粒，这表明粪-口传播不是主要途径。但是，需要进行更大规模的研究，尤其是在新的病毒变异出现的情况下。
