气相色谱-质谱联用分析及[具体物质]的镇痛、抗炎、解热和抗高血糖潜力
Gas chromatography-mass spectrometry profiling and analgesic, anti-inflammatory, antipyretic, and antihyperglycemic potentials of .
作者信息
Mahmoud Amany Hamouda, Samy Mamdouh Nabil, Wanas Amira Samir, Kamel Mohamed Salah
机构信息
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
出版信息
Iran J Basic Med Sci. 2021 May;24(5):641-649. doi: 10.22038/ijbms.2021.53390.12016.
OBJECTIVES
The present study determines the chemical constituents of using gas chromatography-mass spectrometry (GC-MS) and its different activities.
MATERIALS AND METHODS
Air-dried powdered leaves of were extracted by 95% methanol and fractionated consecutively with petroleum ether, chloroform, and ethyl acetate. The saponifiable matter, EtOAc and aqueous fractions were subjected to GC-MS. The analgesic, anti-inflammatory, antipyretic, and antihyperglycemic properties of extracts, different fractions, and crude polysaccharide were determined by hot plate, carrageenan-induced paw edema, yeast-induced pyrexia, and alloxan-induced hyperglycemia methods, respectively.
RESULTS
Fourteen fatty acid methyl esters were identified in GC-MS-based profiling of the saponifiable matter. Alongside, 13 compounds were determined from EtOAc fraction and 6 compounds from the aqueous fraction of leaves. The ethyl acetate fraction and total stem extract displayed high anti-inflammatory potential with percentage of paw edema reduction by 48.99 and 47.54 %, respectively, compared with that of indomethacin (42.90 %). The ethyl acetate fraction and total stem extract revealed the highest analgesic activity with 137.95 and 137.12 % percent of protection against external stimulus, respectively. Investigation of antipyretic efficiency showed the total stem extract and crude polysaccharides attained normal temperature after 3 hr, which was very close to that of acetylsalicylic acid. The total leaf and stem extracts displayed significant antihyperglycemic activity with significant reduction in the level of blood glucose by 76.67 and 59.05 %, respectively.
CONCLUSION
had analgesic, anti-inflammatory, antipyretic, and antihyperglycemic properties, which refer to its bioactive metabolites.
目的
本研究采用气相色谱 - 质谱联用（GC - MS）法测定[植物名称未给出]的化学成分及其不同活性。
材料与方法
将[植物名称未给出]的风干叶粉用95%甲醇提取，并依次用石油醚、氯仿和乙酸乙酯进行分离。对可皂化物质、乙酸乙酯和水相部分进行GC - MS分析。分别采用热板法、角叉菜胶诱导的爪肿胀法、酵母诱导的发热法和四氧嘧啶诱导的高血糖法测定提取物、不同分离部分和粗多糖的镇痛、抗炎、解热和抗高血糖特性。
结果
在基于GC - MS的可皂化物质分析中鉴定出14种脂肪酸甲酯。此外，从[植物名称未给出]叶的乙酸乙酯部分测定出13种化合物，水相部分测定出6种化合物。乙酸乙酯部分和茎总提取物显示出较高的抗炎潜力，与吲哚美辛（42.90%）相比，爪肿胀减少百分比分别为48.99%和47.54%。乙酸乙酯部分和茎总提取物显示出最高的镇痛活性，对外部刺激的保护率分别为137.95%和137.12%。解热效率研究表明，茎总提取物和粗多糖在3小时后恢复到正常体温，这与乙酰水杨酸非常接近。叶和茎总提取物显示出显著的抗高血糖活性，血糖水平分别显著降低76.67%和59.05%。
结论
[植物名称未给出]具有镇痛、抗炎、解热和抗高血糖特性，这归因于其生物活性代谢产物。
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