Guzzetti Lorenzo, Panzeri Davide, Ulaszewska Marynka, Sacco Grazia, Forcella Matilde, Fusi Paola, Tommasi Nicola, Fiorini Andrea, Campone Luca, Labra Massimo
Department of Biotechnology and Bioscience, University of Milan-Bicocca, Milan, Italy.
Department of Food Quality and Nutrition, Research and Innovation Center, Edmund Mach Foundation, Trento, Italy.
Front Nutr. 2021 Jul 1;8:667812. doi: 10.3389/fnut.2021.667812. eCollection 2021.
L. is an African leafy vegetable of high nutritional interest. To assess its agricultural suitability to sustainable cultivation conditions and its potential benefits for human nutrition, its phytochemical content in response to conservation agriculture practices [i.e., no-tillage (NT) and cover crop maintenance] and low water regime were evaluated and compared with response under conventional agriculture management. Hydric stress and NT did not affect the content of antioxidant metabolites, compared to conventional agricultural practices. In both conditions, leaves were found to be a great source of phenolic compounds. The effect of these phenolic fractions was assessed on two colon cell phenotypes to evaluate putative nutraceutical properties. Polyphenol-enriched extracts (PEEs) displayed selective cytotoxic activities against tumor Caco-2 cells but not on the healthy CCD841 line. PEEs were able to trigger oxidative stress and to inhibit the activity of glutathione-independent antioxidant enzymes on Caco-2 cells. showed to be a promising crop for improving both agricultural sustainability and health benefits due to the great amount of antioxidant compounds in leaves, whose occurrence is not altered by stressful farming conditions. Given its high adaptability, the cultivation of this crop is therefore recommendable also in the Mediterranean Basin.
L.是一种具有很高营养价值的非洲叶菜类蔬菜。为评估其对可持续种植条件的农业适宜性及其对人类营养的潜在益处，对其在保护性农业实践（即免耕（NT）和覆盖作物维持）及低水分条件下的植物化学成分进行了评估，并与传统农业管理下的反应进行了比较。与传统农业实践相比，水分胁迫和免耕并未影响抗氧化代谢物的含量。在这两种情况下，叶片都是酚类化合物的重要来源。评估了这些酚类组分对两种结肠细胞表型的影响，以评估其潜在的营养保健特性。富含多酚的提取物（PEEs）对肿瘤Caco-2细胞具有选择性细胞毒性活性，但对健康的CCD841细胞系无此活性。PEEs能够引发氧化应激并抑制Caco-2细胞中不依赖谷胱甘肽的抗氧化酶的活性。由于叶片中含有大量抗氧化化合物，其含量不会因恶劣的种植条件而改变，因此L.显示出有望同时改善农业可持续性和健康效益的作物。鉴于其高适应性，因此在地中海盆地也推荐种植这种作物。
