Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt; Department of Pharmaceutics, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo 11562, Egypt; Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt.
Biomed Pharmacother. 2021 Jun;138:111537. doi: 10.1016/j.biopha.2021.111537. Epub 2021 Apr 5.
Aging of the skin is a complicated bioprocess that is affected by constant exposure to ultraviolet irradiation. The application of herbal-based anti-aging creams is still the best choice for treatment. In the present study, Citrus sinensis L. fruit peels ethanolic extract (CSPE) was formulated into lipid nanoparticles (LNPs) anti-aging cream. Eight different formulations of CSEP-LNPs were prepared and optimized using 2 full factorial designs. In vivo antiaging effect of the best formula was tested in Swiss albino mice where photo-aging was induced by exposure to UV radiation. HPLC-QToF-MS/MS metabolic profiling of CSPE led to the identification of twenty-nine metabolites. CSPE was standardized to a hesperidin content of 15.53 ± 0.152 mg% using RP-HPLC. It was suggested that the optimized formulation (F7) had (245 nm) particle size, (91.065%) EE, and (91.385%) occlusive effect with a spherical and smooth surface. The visible appearance of UV-induced photoaging in mice was significantly improved after topical application on CSPE-NLC cream for 5 weeks, levels of collagen and SOD were significantly increased in CSPE- NLC group, while levels of PGE2, COX2, JNK, MDA, and elastin was reduced. Finally, The prepared anti-aging CSPE-NLC cream represents a safe, convenient, and promising skincare cosmetic product.
皮肤老化是一个复杂的生物过程，受到持续暴露在紫外线下的影响。应用基于草药的抗衰老霜仍然是治疗的最佳选择。在本研究中，将柑橘果皮乙醇提取物（CSPE）制成脂质纳米粒（LNPs）抗衰老霜。使用 2 个全因子设计制备并优化了 8 种不同的 CSPE-LNPs 配方。在瑞士白化小鼠中测试了最佳配方的体内抗衰老效果，这些小鼠通过暴露在紫外线下诱导光老化。CSPE 的 HPLC-QToF-MS/MS 代谢谱分析导致鉴定出 29 种代谢物。CSPE 采用反相高效液相色谱法标准化为柚皮苷含量为 15.53±0.152mg%。研究表明，优化后的配方（F7）具有（245nm）粒径、（91.065%）EE 和（91.385%）封闭效果，表面呈球形且光滑。在 CSPE-NLC 霜剂局部应用 5 周后，可显著改善小鼠 UV 诱导的光老化的可见外观，CSPE-NLC 组中胶原蛋白和 SOD 的水平显著增加，而 PGE2、COX2、JNK、MDA 和弹性蛋白的水平降低。最后，所制备的具有抗衰老作用的 CSPE-NLC 霜剂代表了一种安全、方便且有前景的护肤化妆品产品。
