Division of Oncology, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
Department of Pathology, Semmelweis University, Hungary.
Clin Transl Med. 2021 Jul;11(7):e473. doi: 10.1002/ctm2.473.
The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in-depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectrum of diverse, surgically isolated melanoma tumors originating from multiple body locations. More than 15,500 proteoforms were identified by mass spectrometry, from which chromosomal and subcellular localization was annotated within both primary and metastatic melanoma. The data generated by global proteomic experiments covered 72% of the proteins identified in the recently reported high stringency blueprint of the human proteome. This study contributes to the NIH Cancer Moonshot initiative combining detailed histopathological presentation with the molecular characterization for 505 melanoma tumor samples, localized in 26 organs from 232 patients.
MM500 研究旨在绘制恶性黑色素瘤肿瘤样本中的蛋白质水平图谱，重点是深入的组织病理学与蛋白质组学特征相结合。通过质谱法鉴定了来自多个身体部位的广泛多样的手术分离黑色素瘤肿瘤的蛋白质水平和定位。其中，超过 15500 种蛋白质被鉴定，其中包括在原发性和转移性黑色素瘤中注释的染色体和亚细胞定位。通过全局蛋白质组学实验生成的数据涵盖了最近报道的人类蛋白质组高严谨性蓝图中鉴定出的蛋白质的 72%。这项研究为 NIH 癌症登月计划做出了贡献，该计划将详细的组织病理学表现与 505 个黑色素瘤肿瘤样本的分子特征相结合，这些样本分布在 232 名患者的 26 个器官中。
