Yang Le, Li Xuemei, Xie Meihua, Bai Na, Yang Jiangliu, Jiang Kexin, Zhang Ke-Qin, Yang Jinkui
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, and Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan University, Kunming 650032, China.
School of Life Science, Yunnan University, Kunming 650032, China.
iScience. 2021 Jul 8;24(8):102820. doi: 10.1016/j.isci.2021.102820. eCollection 2021 Aug 20.
The nematode-trapping fungi are ideal agents for controlling pathogenic nematodes. is a representative species of the same, producing traps for nematode predation. Here, three orthologous Ras GTPases (Ras2, Ras3, and Rheb) were characterized in . Our results indicate that they play pleiotropic roles in regulating the mycelial growth, conidiation, stress resistance, and pathogenicity of Furthermore, deletion of and significantly affected the mitochondrial activity, reactive oxygen species levels, lipid storage, and autophagy. Transcriptome analyses of Δ mutant revealed that many repressed genes were associated with signal transduction, energy production, and carbohydrate transport and metabolism. Moreover, metabolic profile analyses showed that AoRas2 and AoRheb affect the biosynthesis of secondary metabolites in . Collectively, these findings provide an in-depth insight into the essential roles of Ras GTPases in vegetative growth, development, and pathogenicity and highlight their importance in the lifestyle switch of the nematode-trapping fungi.
捕食线虫真菌是控制致病线虫的理想媒介。[具体物种名称]是其中的一个代表性物种，能产生用于捕食线虫的陷阱。在此，对[具体物种名称]中的三个直系同源Ras GTP酶（Ras2、Ras3和Rheb）进行了表征。我们的结果表明，它们在调节[具体物种名称]的菌丝生长、产孢、抗逆性和致病性方面发挥着多效性作用。此外，Ras2和Rheb的缺失显著影响了线粒体活性、活性氧水平、脂质储存和自噬。对Δ突变体的转录组分析表明，许多受抑制的基因与信号转导、能量产生以及碳水化合物运输和代谢有关。此外，代谢谱分析表明，AoRas2和AoRheb影响[具体物种名称]中次生代谢物的生物合成。总的来说，这些发现深入揭示了Ras GTP酶在营养生长、发育和致病性中的重要作用，并突出了它们在捕食线虫真菌生活方式转变中的重要性。
