Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Raw Material Technology Center of Guangxi Tobacco, Nanning 530000, China.
Bioresour Technol. 2021 Nov;340:125668. doi: 10.1016/j.biortech.2021.125668. Epub 2021 Jul 27.
This study evaluated the effects of biocontrol Bacillus and fermenting bacteria addition on the microbial community, metabolic functions and antibiotic resistance genes (ARGs) of new prickly ash seed oil meal (PSOM)-biochar composting. The results showed that the addition of Bacillus subtilis and fermentation bacteria significantly increased the NH-N, bacterial abundance and fungal diversity of compost while decreasing the relative abundances (RAs) of carbon metabolism genes in mature compost. NH-N was significantly correlated with microbial abundance and diversity, and its increase was closely related to microbial amino acid metabolism. The addition of biocontrol and fermenting bacteria changed the RAs of ARGs, which was caused by changes in the potential hosts Proteobacteria, Bacteroidota and Firmicutes in the compost. Consequently, adding Bacillus and fermenting bacteria into PSOM to make composting was suggested as an effective method to promote nutrient transformation, regulate microbial activity and decrease RAs of tetracycline and vancomycin ARGs.
本研究评估了生防芽孢杆菌和发酵菌添加对新花椒籽粕（PSOM）-生物炭堆肥中微生物群落、代谢功能和抗生素抗性基因（ARGs）的影响。结果表明，枯草芽孢杆菌和发酵菌的添加显著增加了堆肥中 NH-N、细菌丰度和真菌多样性，而降低了成熟堆肥中碳代谢基因的相对丰度（RAs）。NH-N 与微生物丰度和多样性呈显著正相关，其增加与微生物氨基酸代谢密切相关。生防和发酵菌的添加改变了 ARGs 的 RAs，这是由于堆肥中潜在宿主变形菌门、拟杆菌门和厚壁菌门的变化引起的。因此，将芽孢杆菌和发酵菌添加到 PSOM 中进行堆肥被认为是一种有效促进养分转化、调节微生物活性和降低四环素和万古霉素 ARGs 的 RAs 的方法。
