Servicio Autónomo Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.
Instituto Venezolano de Investigaciones Científicas (IVIC), Los Teques, Venezuela.
Int J Infect Dis. 2021 Oct;111:261-266. doi: 10.1016/j.ijid.2021.07.070. Epub 2021 Jul 31.
We evaluated the immunoglobulin (Ig) G antibody response against the nucleocapsid protein (NP) and the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 in a cohort of 86 individuals in Venezuela, before and after receiving the Sputnik V vaccine.
Antibody responses against NP and RBD were determined with an enzyme-linked immunosorbent assay just before, 3 weeks after the first, and 6 weeks after the second dose of the vaccine.
Before vaccination, 59 individuals were seronegative, and 27 seropositive for NP and/or RBD. Of the seronegative cohort, 42% did not develop an IgG immune response against RBD after the first vaccine dose, but 100% had a strong IgG response after 2 doses. All seropositive individuals developed a strong IgG antibody response against RBD after the first vaccine dose, with antibody levels ∼40% higher than seronegative individuals who had received 2 doses. Previously seropositive subjects showed no significant increase in IgG antibody response against RBD after the second vaccine dose.
We demonstrate that 2 doses of the Sputnik V vaccine triggered antibody response in all study individuals. The second Sputnik V dose had no impact on IgG response for those seropositive for SARS-CoV-2 antigens before vaccination.
我们评估了 86 名委内瑞拉个体在接种卫星 V 疫苗前后针对 SARS-CoV-2 的核衣壳蛋白（NP）和刺突蛋白受体结合域（RBD）的 IgG 抗体反应。
在接种疫苗前、第一剂后 3 周和第二剂后 6 周，用酶联免疫吸附试验测定针对 NP 和 RBD 的抗体反应。
接种疫苗前，59 人血清阴性，27 人 NP 和/或 RBD 血清阳性。在血清阴性队列中，42%的人在第一剂疫苗后未产生针对 RBD 的 IgG 免疫反应，但在接种 2 剂后 100%的人产生了强烈的 IgG 反应。所有血清阳性个体在接种第一剂疫苗后均产生针对 RBD 的强烈 IgG 抗体反应，抗体水平比接种 2 剂的血清阴性个体高约 40%。先前血清阳性的个体在接种第二剂疫苗后，针对 RBD 的 IgG 抗体反应没有显著增加。
我们证明，2 剂卫星 V 疫苗可触发所有研究个体的抗体反应。对于接种疫苗前 SARS-CoV-2 抗原血清阳性的个体，第二剂卫星 V 疫苗对 IgG 反应没有影响。
