成年人血清中 Sirtuin6 的年龄相关性变化。
Age-related changes of human serum Sirtuin6 in adults.
机构信息
Department of Endocrinology, Institute of geriatric medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, 39 Lake Road, East Lake Ecological Scenic, Wuhan, 430077, Hubei Province, China.
出版信息
BMC Geriatr. 2021 Aug 4;21(1):452. doi: 10.1186/s12877-021-02399-0.
BACKGROUND
Aging is a natural life process and with an aging population, age-related diseases (e.g. type 2 diabetes mellitus (T2DM), atherosclerosis-based cardiovascular diseases) are the primary mortality cause in older adults. Telomerase is often used as an aging biomarker. Detection and characterization of novel biomarkers can help in a more specific and sensitive identification of a person's aging status. Also, this could help in age-related diseases early prevent, ultimately prolonging the population's life span. Sirtuin 6 (Sirt6) - a member of the Sirtuins NAD-dependent histone deacetylases family - is mainly intracellularly expressed, and is reported to be involved in the regulation of aging and aging-related diseases. Whether serum Sirt6 is correlated with aging and could be used as an aging biomarker is unknown. In the present study, we aimed to investigate the age-related Sirt6 changes in the serum of human adults.
METHODS
Participants were divided into three groups according to age: 20-30 years (Young); 45-55 years (Middle-aged); and ≥ 70 years (Old). The Sirt6 and telomerase serum concentrations were determined by ELISA. The Sirt6 and human telomerase reverse transcriptase (hTERT) expression in vessels from amputated human lower limbs were analyzed using real-time quantitative PCR (RT-qPCR) and immunohistochemical staining. The relationships between variables were evaluated by Pearson correlation analysis.
RESULTS
The Sirt6 and telomerase serum levels reduced with an increase in age. A similar tendency was observed for Sirt6 and hTERT in the vessel. Serum levels of Sirt6 were higher in females compared with males. Pearson's regression analysis revealed that the Sirt6 serum level positively correlated with telomerase (r = 0.5743) and both were significantly negatively correlated with age (r = - 0.5830 and r = - 0.5993, respectively).
CONCLUSIONS
We reported a negative correlation between serum Sirt6 concentration and aging in human beings. Therefore, the Sirt6 serum level is a potential sex-specific aging marker.
背景
衰老是一个自然的生命过程，随着人口老龄化，与年龄相关的疾病（例如 2 型糖尿病（T2DM）、基于动脉粥样硬化的心血管疾病）成为老年人的主要死亡原因。端粒酶通常被用作衰老生物标志物。检测和表征新的生物标志物有助于更准确和敏感地识别一个人的衰老状态。此外，这有助于早期预防与年龄相关的疾病，最终延长人口寿命。Sirtuin 6（Sirt6）- 一种 NAD 依赖性组蛋白去乙酰化酶家族的 Sirtuins 成员 - 主要在细胞内表达，据报道与衰老和与衰老相关的疾病的调节有关。血清 Sirt6 是否与衰老相关，是否可作为衰老生物标志物尚不清楚。在本研究中，我们旨在研究人类成年人血清中与年龄相关的 Sirt6 变化。
方法
根据年龄将参与者分为三组：20-30 岁（年轻）；45-55 岁（中年）；和≥70 岁（老年）。通过 ELISA 测定 Sirt6 和端粒酶血清浓度。使用实时定量 PCR（RT-qPCR）和免疫组织化学染色分析从截肢的人类下肢血管中 Sirt6 和人端粒酶逆转录酶（hTERT）的表达。通过 Pearson 相关分析评估变量之间的关系。
结果
随着年龄的增长，Sirt6 和端粒酶的血清水平降低。血管中的 Sirt6 和 hTERT 也呈现出类似的趋势。与男性相比，女性的血清 Sirt6 水平更高。Pearson 回归分析显示，Sirt6 血清水平与端粒酶呈正相关（r=0.5743），且均与年龄呈显著负相关（r=-0.5830 和 r=-0.5993）。
结论
我们报告了人类血清 Sirt6 浓度与衰老之间的负相关关系。因此，Sirt6 血清水平是一种潜在的性别特异性衰老标志物。
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