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InfoBoss Research Center, 301 room, Haeun Bldg., 670, Seolleung-ro, Gangnam-gu, Seoul, 07766, Korea.
Sci Rep. 2021 Aug 16;11(1):16578. doi: 10.1038/s41598-021-95940-5.
GATA transcription factors (TFs) are widespread eukaryotic regulators whose DNA-binding domain is a class IV zinc finger motif (CXCXCXC) followed by a basic region. We identified 262 GATA genes (389 GATA TFs) from seven Populus genomes using the pipeline of GATA-TFDB. Alternative splicing forms of Populus GATA genes exhibit dynamics of GATA gene structures including partial or full loss of GATA domain and additional domains. Subfamily III of Populus GATA genes display lack CCT and/or TIFY domains. 21 Populus GATA gene clusters (PCs) were defined in the phylogenetic tree of GATA domains, suggesting the possibility of subfunctionalization and neofunctionalization. Expression analysis of Populus GATA genes identified the five PCs displaying tissue-specific expression, providing the clues of their biological functions. Amino acid patterns of Populus GATA motifs display well conserved manner of Populus GATA genes. The five Populus GATA genes were predicted as membrane-bound GATA TFs. Biased chromosomal distributions of GATA genes of three Populus species. Our comparative analysis approaches of the Populus GATA genes will be a cornerstone to understand various plant TF characteristics including evolutionary insights.
GATA 转录因子（TFs）是广泛存在的真核生物调节剂，其 DNA 结合域是一个 IV 类锌指基序（CXCXCXC），后面跟着一个碱性区域。我们使用 GATA-TFDB 管道从七个杨树基因组中鉴定了 262 个 GATA 基因（389 个 GATA TFs）。杨树 GATA 基因的选择性剪接形式表现出 GATA 基因结构的动态，包括 GATA 结构域的部分或完全缺失以及额外的结构域。杨树 GATA 基因的亚家族 III 缺乏 CCT 和/或 TIFY 结构域。在 GATA 结构域的系统发育树中定义了 21 个杨树 GATA 基因簇（PCs），这表明存在亚功能化和新功能化的可能性。杨树 GATA 基因的表达分析确定了五个显示组织特异性表达的 PCs，为它们的生物学功能提供了线索。杨树 GATA 基序的氨基酸模式显示出杨树 GATA 基因的高度保守方式。预测这五个杨树 GATA 基因是膜结合 GATA TFs。三种杨树物种的 GATA 基因的染色体偏性分布。我们对杨树 GATA 基因的比较分析方法将是理解包括进化见解在内的各种植物 TF 特征的基石。
