Morimoto Yusuke V, Namba Keiichi, Minamino Tohru
Quantitative Biology Center, RIKEN, Suita, Osaka, Japan.
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Bio Protoc. 2017 Jan 5;7(1):e2093. doi: 10.21769/BioProtoc.2093.
Bacteria such as and swim in liquid media using the bacterial flagella. The flagellum consists of the basal body (rotary motor), the hook (universal joint) and the filament (helical screw). Since mutants with a defect in flagellar assembly and function cannot swim smoothly, motility assay is an easy way to characterize flagellar mutants. Here, we describe how to measure free-swimming speeds of motile cells in liquid media. Free-swimming behavior under a microscope shows a significant variation among bacterial cells.
诸如 和 之类的细菌利用细菌鞭毛在液体培养基中游动。鞭毛由基体（旋转马达）、钩形结构（万向节）和鞭毛丝（螺旋螺杆）组成。由于鞭毛组装和功能有缺陷的突变体不能顺畅游动，运动性测定是表征鞭毛突变体的一种简便方法。在此，我们描述如何测量液体培养基中 运动细胞的自由游动速度。显微镜下的自由游动行为在细菌细胞之间显示出显著差异。
