LI-COR Biosciences, Lincoln, Nebraska, USA.
School of Natural Resources, University of Nebraska, Lincoln, Nebraska, USA.
Plant Cell Environ. 2021 Nov;44(11):3509-3523. doi: 10.1111/pce.14178. Epub 2021 Sep 15.
Leaf level gas exchange is a widely used technique that provides real-time measurement of leaf physiological properties, including CO assimilation (A), stomatal conductance to water vapour (g ) and intercellular CO (C ). Modern open-path gas exchange systems offer greater portability than the laboratory-built systems of the past and take advantage of high-precision infrared gas analyzers and optimized system design. However, the basic measurement paradigm has long required steady-state conditions for accurate measurement. For CO response curves, this requirement has meant that each point on the curve needs 1-3 min and a full response curve generally requires 20-35 min to obtain a sufficient number of points to estimate parameters such as the maximum velocity of carboxylation (V ) and the maximum rate of electron transport (J ). For survey measurements, the steady-state requirement has meant that accurate measurement of assimilation has required about 1-2 min. However, steady-state conditions are not a strict prerequisite for accurate gas exchange measurements. Here, we present a new method, termed dynamic assimilation, that is based on first principles and allows for more rapid gas exchange measurements, helping to make the technique more useful for high throughput applications.
叶片水平气体交换是一种广泛应用的技术，可实时测量叶片的生理特性，包括 CO 同化（A）、水蒸气的气孔导度（g）和细胞间 CO（C）。现代开路气体交换系统比过去的实验室构建系统具有更高的便携性，利用高精度的红外气体分析仪和优化的系统设计。然而，基本的测量范式长期以来一直要求稳定状态条件才能进行准确测量。对于 CO 响应曲线，这一要求意味着曲线的每个点都需要 1-3 分钟，而完整的响应曲线通常需要 20-35 分钟才能获得足够数量的点来估计参数，如羧化的最大速度（V）和电子传递的最大速率（J）。对于调查测量，稳定状态的要求意味着同化的准确测量需要大约 1-2 分钟。然而，稳定状态并不是准确气体交换测量的严格前提条件。在这里，我们提出了一种新的方法，称为动态同化，它基于第一性原理，允许更快速的气体交换测量，有助于使该技术更适用于高通量应用。
