Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan Province, P. R. China.
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, Hunan Province, P. R. China.
Biomater Sci. 2021 Oct 12;9(20):6691-6717. doi: 10.1039/d1bm01057c.
Engineering cellular membranes with functional molecules provides an attractive strategy to manipulate cellular behaviors and functionalities. Currently, synthetic deoxyribonucleic acid (DNA) has emerged as a promising molecular tool to engineer cellular membranes for biomedical applications due to its molecular recognition and programmable properties. In this review, we summarized the recent advances in anchoring DNA on the cellular membranes and their applications. The strategies for anchoring DNA on cell membranes were summarized. Then their applications, such as immune response activation, receptor oligomerization regulation, membrane structure mimicking, cell-surface biosensing, and construction of cell clusters, were listed. The DNA-enabled intelligent systems which were able to sense stimuli such as DNA strands, light, and metal ions were highlighted. Finally, insights regarding the remaining challenges and possible future directions were provided.
用功能分子工程化细胞膜为操控细胞行为和功能提供了一种有吸引力的策略。目前，由于其分子识别和可编程特性，合成脱氧核糖核酸（DNA）已成为用于生物医学应用的工程化细胞膜的一种很有前途的分子工具。在这篇综述中，我们总结了近年来在将 DNA 锚定在细胞膜上及其应用方面的进展。总结了将 DNA 锚定在细胞膜上的策略。然后列出了它们的应用，如免疫反应激活、受体寡聚化调节、模拟膜结构、细胞表面生物传感和细胞簇的构建。重点介绍了能够感应 DNA 链、光和金属离子等刺激的 DNA 功能智能系统。最后，对存在的挑战和可能的未来方向提供了一些见解。
