Wang Juncai, Chen Xunfeng, Chu Shaohua, You Yimin, Chi Yaowei, Wang Renyuan, Yang Xijia, Hayat Kashif, Zhang Dan, Zhou Pei
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Areas, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Areas, Shanghai Jiao Tong University, Shanghai 200240, China.
J Hazard Mater. 2022 Feb 5;423(Pt B):127168. doi: 10.1016/j.jhazmat.2021.127168. Epub 2021 Sep 11.
Cadmium (Cd) triggers molecular alterations in plants, perturbs metabolites and damages plant growth. Therefore, understanding the molecular mechanism underlying the Cd tolerance in plants is necessary for assessing the persistent environmental impact of Cd. In this study, Solanum nigrum was selected as the test plant to investigate changes in biomass, Cd translocation, cell ultrastructure, metabolites and genes under hydroponic conditions. The results showed that the plant biomass was significantly decreased under Cd stress, and the plant has a stronger Cd transport capability. Transmission electron microscopy revealed that increased Cd concentration gradually damaged the plant organs (roots, stems and leaves) cell ultrastructure, as evidenced by swollen chloroplasts and deformed cell walls. Additionally, metabolomics analyses revealed that Cd stress mainly affected seven metabolism pathways, including 19 differentially expressed metabolites (DEMs). Moreover, 3908 common differentially expressed genes (DEGs, 1049 upregulated and 2859 downregulated) were identified via RNA-seq among five Cd treatments. Meanwhile, conjoint analysis found several DEGs and DEMs, including laccase, peroxidase, D-fructose, and cellobiose etc., are associated with cell wall biosynthesis, implying the cell wall biosynthesis pathway plays a critical role in Cd detoxification. Our comprehensive investigation using multiple approaches provides a molecular-scale perspective on plant response to Cd stress.
镉（Cd）会引发植物的分子变化，扰乱代谢物并损害植物生长。因此，了解植物对镉耐受性的分子机制对于评估镉对环境的持续影响至关重要。在本研究中，选择龙葵作为试验植物，研究水培条件下生物量、镉转运、细胞超微结构、代谢物和基因的变化。结果表明，镉胁迫下植物生物量显著降低，且植物具有较强的镉转运能力。透射电子显微镜显示，镉浓度升高逐渐破坏了植物器官（根、茎和叶）的细胞超微结构，叶绿体肿胀和细胞壁变形证明了这一点。此外，代谢组学分析表明，镉胁迫主要影响七条代谢途径，包括19种差异表达代谢物（DEM）。此外，通过RNA测序在五种镉处理中鉴定出3908个常见的差异表达基因（DEG，1049个上调和2859个下调）。同时，联合分析发现几种DEG和DEM，包括漆酶、过氧化物酶、D-果糖和纤维二糖等，与细胞壁生物合成有关，这意味着细胞壁生物合成途径在镉解毒中起关键作用。我们使用多种方法进行的综合研究为植物对镉胁迫的响应提供了分子层面的视角。
