Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy; Center for Translational Research on Autoimmune and Allergic Diseases, University of Piemonte Orientale, Novara, Italy.
Department of Health Sciences, University of Piemonte Orientale, Novara, Italy.
Anal Chim Acta. 2021 Sep 22;1179:338841. doi: 10.1016/j.aca.2021.338841. Epub 2021 Jul 7.
The intestinal microbiota is composed of a large number of different bacteria that produce a variety of metabolites. Colorectal cancer, which typically develops from adenomatous polyps, is highly influenced by microbiota. Since a variety of molecular changes may occur as these polyps transform from benign tumor to malignant carcinoma, the ability to study the microbiota-produced metabolites can lead to new discoveries about the development and progression of this cancer. However, to address the complexity of the microbiota-produced molecules, novel methods are needed. To this aim, in the present work, we developed a high-throughput metabolomics method to capture the metabolic complexity of the microbiota metabolome adherent to adenomatous polyps and adenocarcinoma. For the first time, the method enables the simultaneous quantification of almost 300 metabolites, while preserving the integrity of the original sample. The metabolomics approach was analytically validated and had excellent performances in terms of recovery, linearity, specificity, intra- and inter-day precision, limits of detection, and quantification. Furthermore, the clinical potential of the method was demonstrated in adenoma collected for a colorectal adenoma study.
肠道微生物群由大量不同的细菌组成，这些细菌会产生多种代谢物。结直肠癌通常由腺瘤性息肉发展而来，其受微生物群的影响很大。由于这些息肉从良性肿瘤转变为恶性癌时可能会发生多种分子变化，因此研究微生物群产生的代谢物的能力可以为这种癌症的发展和进展提供新的发现。然而，为了解决微生物群产生的分子的复杂性，需要新的方法。为此，在本工作中，我们开发了一种高通量代谢组学方法，以捕捉黏附于腺瘤性息肉和腺癌的微生物组代谢物的代谢复杂性。该方法首次能够同时定量近 300 种代谢物，同时保持原始样本的完整性。该代谢组学方法经过了分析验证，在回收率、线性度、特异性、日内和日间精密度、检测限和定量限方面均具有优异的性能。此外，该方法在结直肠腺瘤研究中收集的腺瘤中的临床应用潜力也得到了证明。
